NDLI: Binding Interactions of Toluidine Blue O with Escherichia Coli DNA: Formation of Bridged Structure

Content Provider	Springer Nature Link
Author	Ilanchelian, Malaichamy Ramaraj, Ramasamy
Copyright Year	2011
Abstract	The interaction(s) of Toluidine Blue O (TBO) with DNA was investigated using absorption and emission spectral methods. The binding of TBO with DNA was understood from the observed hypochromism in the absorption spectra and decrease in the emission intensity of TBO. From the absorption and emission spectral data, two binding constants were estimated for the binding of TBO with DNA. Based on the binding constant values both intercalative and electrostatic interactions of TBO with DNA were suggested. The TBO-DNA binding constant values reveal that the electrostatic interaction of TBO with DNA is weaker than the intercalative interaction. The emission quenching and salt effect studies showed that the TBO was partially intercalated with DNA. The two modes of binding between TBO and DNA may lead to the formation of bridging of a pair of DNA duplexes by TBO molecule. The electrostatic interaction is more important for the formation of the bridged structure of TBO with DNA. This was verified by studying the interaction of an anionic dye, Eosin Y (EY).
Starting Page	1439
Ending Page	1453
Page Count	15
File Format	PDF
ISSN	10530509
Journal	Journal of Fluorescence
Volume Number	21
Issue Number	4
e-ISSN	15734994
Language	English
Publisher	Springer US
Publisher Date	2011-02-23
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Bridged structure Partial intercalation Electrostatic binding Toluidine blue O Biochemistry Biotechnology Biophysics and Biological Physics Biomedicine general Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Sociology and Political Science Spectroscopy Law Biochemistry Clinical Biochemistry Clinical Psychology

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