NDLI: Hydrophilic Labeling Reagents of Dipyrrylmethene-BF$_{2}$ Dyes for Two-Photon Excited Fluorometry: Syntheses and Photophysical Characterization

Content Provider	Springer Nature Link
Author	Meltola, Niko J. Wahlroos, Rina Soini, Aleksi E.
Copyright Year	2004
Abstract	Recently introduced bioaffinity assay technology, ArcDia™ TPX, is based on two-photon excited fluorescence (TPE) and it enables separation-free ultra-sensitive immunoassays from microvolumes. Here we present syntheses of novel two-photon excitable fluorescent labeling reagents which have been specially designed to be used as label molecules in the ArcDia™ TPX assay technique. The labeling reagents are based on dipyrrylmetheneboron difluoride (dipyrrylmethene-BF$_{2}$) chromophore, which have been substituted with aryl, heteroaryl or arylalkenyl chemical groups to extend the π-electron conjugation. These substitutions results in a series of dipyrrylmethene-BF$_{2}$ fluorophores with different photophysical properties. Dipyrrylmethene-BF$_{2}$ fluorophores have been further substituted with a dipeptide linker unit and finally activated as succinimidyl esters to enable specific coupling with primary amino groups. The dipeptide linker serves as a spacer arm between the label and a target, and enhances the solubility of the label in aqueous solutions. Study of the chemical and photophysical performance of the new labeling reagents is described. The new labeling reagents exhibit high fluorescence quantum yields, and molar absorption coefficients. The results show that the new labels with the hydrophilic dipeptide linker unit provide large two-photon excitation cross-sections, high fluorescence quantum efficiency and good solubility in aqueous solutions. The results suggest that the novel dipyrrylmethene-BF$_{2}$ labels are highly applicable to bioaffinity assays based on two-photon excitation of fluorescence.
Starting Page	635
Ending Page	647
Page Count	13
File Format	PDF
ISSN	10530509
Journal	Journal of Fluorescence
Volume Number	14
Issue Number	5
e-ISSN	15734994
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2004-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Analytical Chemistry Biochemistry Biophysics/Biomedical Physics
Content Type	Text
Resource Type	Article
Subject	Sociology and Political Science Spectroscopy Law Biochemistry Clinical Biochemistry Clinical Psychology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Syntheses of Novel Dipyrrylmethene-BF$_{2}$ Dyes and Their Performance as Labels in Two-Photon Excited Fluoroimmunoassay

Dipyrrylmetheneboron Difluorides as Labels in Two-Photon Excited Fluorometry. Part I-Immunometric Assays

Dipyrrylmetheneboron Difluorides as Labels in Two-Photon Excited Fluorometry. Part II–Nucleic Acid Hybridization Assays

Two-Photon Excited Fluorescence and Molecular Reorientations in Liquid Solutions

Control of Two-photon Fluorescence of Common Dyes and Conjugated Dyes

Quality Assurance in Fluorometry

Hyper Rayleigh Scattering Yields Improved Response Function in Analysing 2-Photon Excited Fluorescence

Fluorescence of Styryl Dyes-DNA Complexes Induced by Single- and Two-Photon Excitation

New Approach for Fluorescent Peptide Labeling with Pyrylium Cyanine Dyes

Hydrophilic Labeling Reagents of Dipyrrylmethene-BF$_{2}$ Dyes for Two-Photon Excited Fluorometry: Syntheses and Photophysical Characterization

Similar Documents

Syntheses of Novel Dipyrrylmethene-BF$_{2}$ Dyes and Their Performance as Labels in Two-Photon Excited Fluoroimmunoassay

Dipyrrylmetheneboron Difluorides as Labels in Two-Photon Excited Fluorometry. Part I-Immunometric Assays

Dipyrrylmetheneboron Difluorides as Labels in Two-Photon Excited Fluorometry. Part II–Nucleic Acid Hybridization Assays

Two-Photon Excited Fluorescence and Molecular Reorientations in Liquid Solutions

Control of Two-photon Fluorescence of Common Dyes and Conjugated Dyes

Quality Assurance in Fluorometry

Hyper Rayleigh Scattering Yields Improved Response Function in Analysing 2-Photon Excited Fluorescence

Fluorescence of Styryl Dyes-DNA Complexes Induced by Single- and Two-Photon Excitation

New Approach for Fluorescent Peptide Labeling with Pyrylium Cyanine Dyes

Hydrophilic Labeling Reagents of Dipyrrylmethene-BF$_{2}$ Dyes for Two-Photon Excited Fluorometry: Syntheses and Photophysical Characterization