NDLI: Optical Sensor for the Determination of Glucose Based on KIO$

Content Provider	Springer Nature Link
Author	Li, Ming Lee, Sang Hak Bae, Zun Ung Lee, Kwang Pill Park, Yu Chul Lee, Mu Sang
Copyright Year	2004
Abstract	A method to determine glucose using an optical sensor prepared by entrapping glucose oxidase into silica sol-gel column has been developed. The silica sol-gel film was coated on alumina substrate. The optical sensor is based on the chemiluminescence intensity from the reaction of periodate and hydrogen peroxide in K$_{2}$CO$_{3}$ medium. The effect of the ratio of water and alcohol for the preparation of TEOS sol on chemiluminescence intensity was investigated. The effects of pH of enzyme reactor, concentrations of potassium periodate and SDS, and flow rate on the chemiluminescence intensity were studied to find the optimum experimental conditions to determine glucose. The chemiluminescence intensity increased linearly with increasing glucose concentration from 5.0× 10$^{−4}$ M to 1.0× 10$^{−7}$ M and the detection limit was 4.0× 10$^{−8}$ M. Interference effects from some metal ions on chemiluminescence intensity were also investigated.
Starting Page	597
Ending Page	601
Page Count	5
File Format	PDF
ISSN	10530509
Journal	Journal of Fluorescence
Volume Number	14
Issue Number	5
e-ISSN	15734994
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2004-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Analytical Chemistry Biochemistry Biophysics/Biomedical Physics
Content Type	Text
Resource Type	Article
Subject	Sociology and Political Science Spectroscopy Law Biochemistry Clinical Biochemistry Clinical Psychology

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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