NDLI: Impairment of brain mitochondrial functions by β-hemolytic Group B Streptococcus. Effect of cardiolipin and phosphatidylcholine

Content Provider	Springer Nature Link
Author	Macchioni, Lara Fettucciari, Katia Davidescu, Magdalena Vitale, Rita Ponsini, Pamela Rosati, Emanuela Corcelli, Angela Marconi, Pierfrancesco Corazzi, Lanfranco
Copyright Year	2013
Abstract	Group B Streptococcus (GBS) causes severe infection in the central nervous system. In this study, brain mitochondrial function was investigated by simulating infection of isolated mitochondria with GBS, which resulted in loss of mitochondrial activity. The β-hemolysin expressing strains GBS-III-NEM316 and GBS-III-COH31, but not the gGBS-III-COH31 that does not express β-hemolysin, caused dissipation of preformed mitochondrial membrane potential (Δψ$_{m}$). This indicates that β-hemolysin is responsible for decreasing of the reducing power of mitochondria. GBS-III-COH31 interacted with mitochondria causing increase of oxygen consumption, due to uncoupling of respiration, blocking of ATP synthesis, and cytochrome c release outside mitochondria. Moreover, the mitochondrial systems contributing to the control of cellular Ca$^{2+}$ uptake were lost. In spite of these alterations, mitochondrial phospholipid content and composition did not change significantly, as evaluated by MALDI-TOF mass spectrometry. However, exogenous cardiolipin (CL) and dipalmitoylphosphatidylcholine (DPPC) attenuated the uncoupling effect of GBS-III-COH31, although with different mechanisms. CL was effective only when fused to the inner mitochondrial membrane, probably reducing the extent of GBS-induced proton leakage. DPPC, which is not able to fuse with mitochondrial membranes, exerted its effect outside mitochondria, likely by shielding mitochondria against GBS β-hemolysin attack.
Starting Page	519
Ending Page	529
Page Count	11
File Format	PDF
ISSN	0145479X
Journal	Journal of Bioenergetics and Biomembranes
Volume Number	45
Issue Number	6
e-ISSN	15736881
Language	English
Publisher	Springer US
Publisher Date	2013-08-25
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Group B Streptococcus Brain mitochondria Respiratory control Membrane potential Cardiolipin Dipamitoylphosphatidylcholine Bioorganic Chemistry Biochemistry Animal Anatomy / Morphology / Histology Animal Biochemistry Organic Chemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Physiology

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