NDLI: The interplay between plasma membrane and endoplasmic reticulum Ca$^{2+}$ATPases in agonist-induced temporal Ca$^{2+}$ dynamics

Content Provider	Springer Nature Link
Author	Cicek, Figen Amber Ozgur, Ekin Ozge Ozgur, Erol Ugur, Mehmet
Copyright Year	2014
Abstract	A change in the intracellular free Ca$^{2+}$ concentration ([Ca$^{2+}$]$_{i}$) functions as a transmitter for signal transduction and shows a broad temporal pattern. Even genetically homogeneous cell types show different Ca$^{2+}$ response patterns under permanent agonist stimulation. In Ca$^{2+}$ signaling, the dynamics of the Ca$^{2+}$ release from the Ca$^{2+}$ channels during continuous agonist stimulation and the simultaneous effect of the pumps are unclear. In this study, the dynamic interaction of the Ca$^{2+}$ ATPases in the plasma membrane (PMCA) and the endoplasmic reticulum membrane (SERCA) during continuous ACh stimulation is monitored using Fluo-3 and Fura-2 loaded HEK 293 cells. We characterize Ca$^{2+}$ release patterns at the sub-maximal and maximal stimulation doses in the absence of extracellular Ca$^{2+}$. We analyze the responses regarding their types, oscillation frequency and response times. La$^{3+}$ (PMCA blocker) do not change the frequency and time courses in sub-maximal ACh treatment, while with the maximal stimulation oscillation frequency increase as oscillations superimpose on robust release, and response time of [Ca$^{2+}$]$_{i}$ is elongated. A similar effect of La$^{3+}$ is observed in quantal Ca$^{2+}$ release phenomenon. In the presence of CPA, a SERCA blocker, oscillations are completely abolished, but response time does not change. We also observe that during continuous receptor stimulation, Ca$^{2+}$ release do not cease. These data may suggest that Ca$^{2+}$ release continues during agonist stimulation, but SERCA and PMCA form a new steady state and return [Ca$^{2+}$]$_{i}$ to its physiological concentration.
Starting Page	503
Ending Page	510
Page Count	8
File Format	PDF
ISSN	0145479X
Journal	Journal of Bioenergetics and Biomembranes
Volume Number	46
Issue Number	6
e-ISSN	15736881
Language	English
Publisher	Springer US
Publisher Date	2014-10-21
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ca$^{2+}$ signaling SERCA PMCA Ca$^{2+}$ oscillations Quantal release Bioorganic Chemistry Biochemistry Animal Anatomy / Morphology / Histology Animal Biochemistry Organic Chemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Physiology

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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