NDLI: Mitochondrial Ca$^{2+}$ Transients in Cardiac Myocytes During the Excitation–Contraction Cycle: Effects of Pacing and Hormonal Stimulation

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Journal of Bioenergetics and Biomembranes : Volume 30, Issue 2, April 1998

Journal of Bioenergetics and Biomembranes : Volume 30, Issue 1, February 1998

Journal of Bioenergetics and Biomembranes : Volume 29

Mitochondrial Ca$^{2+}$ Transients in Cardiac Myocytes During the Excitation–Contraction Cycle: Effects of Pacing and Hormonal Stimulation

Content Provider	Springer Nature Link
Author	Ohata, Hisayuki Chacon, Enrique Tesfai, Samuel A. Harper, Ian S. Herman, Brian Lemasters, John J.
Copyright Year	1998
Abstract	Using laser scanning confocal microscopy, our objective was to measure mitochondrial, nuclear, and cytosolic free ionized Ca$^{2+}$ in adult rabbit cardiac myocytes loaded with Ca$^{2+}$-indicating fluorophores. When myocytes were loaded with Fluo 3 at 37°C, the fluorophore was loaded extensively into the cytosol and nucleus, but poorly into mitochondria, and Fluo 3 fluorescence transients after field stimulation were confined to the cytosol and nucleus. In contrast, after loading at 4°C, Fluo 3 also entered mitochondria, and large transients of mitochondrial Fluo 3 fluorescence then occurred after stimulation. Isoproterenol (1 μM) increased the magnitude of Ca$^{2+}$ transients and their subsequent rate of decay, an effect more marked in the cytosol and nucleus than in mitochondria. As pacing frequency was increased from 0.5 to 2 Hz, diastolic mitochondrial Ca$^{2+}$ rose markedly in the absence but not in the presence of isoproterenol. Resting Ca$^{2+}$ estimated by Indo 1 ratio imaging using UV/visible laser scanning confocal microscopy was about 200 nM in all compartments. During field stimulation, Ca$^{2+}$ transiently increased to 671, 522, and 487 nM in cytosol, interfibrillar mitochondria, and perinuclear mitochondria, respectively. Isoproterenol increased these respective peak values to 1280, 750, and 573 nM. These results were consistent with those obtained in Fluo 3 experiments. We conclude that rapid mitochondrial Ca$^{2+}$ transients occur during excitation–contraction coupling in adult rabbit cardiac myocytes, which may be important in matching mitochondrial metabolism to myocardial ATP demand during changes in cardiac output.
Starting Page	207
Ending Page	222
Page Count	16
File Format	PDF
ISSN	0145479X
Journal	Journal of Bioenergetics and Biomembranes
Volume Number	30
Issue Number	3
e-ISSN	15736881
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	1998-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Organic Chemistry Bioorganic Chemistry Biochemistry Animal Biochemistry Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Physiology

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