NDLI: Quantification of the bone-related mRNAs at the bone/prosthetic interface

Content Provider	Springer Nature Link
Author	Hunt, J. A. Williams, D. F. Howlett, C. R. Zreiqat, H. Zicat, B.
Copyright Year	1998
Abstract	Aseptic loosening is one of the major reasons for failure of joint prostheses. The periprosthetic tissue has previously been described microscopically; however, little work has been devoted towards quantitating genes expressed by cells at the materials/tissue interface. This study aims to characterize the phenotypic expression of osteoblasts and test the feasibility of quantifying the level of gene expression in periprosthetic tissue sections by combining in situ hybridization and image analysis techniques. There are many factors to consider when quantifying mRNA, in that comparing labeling between different cDNA probes, these should have comparable length and base comparison. The probes should be labeled with the same specific activity, that is the amount of probe to label added is the same, both between different probes and between batches of the same probe. Chromagen color reactions are variable in that the color development is not always linear and more likely follows a sigmoidal curve. Samples should only be compared when it is known that the reaction has been in the linear range. The image analysis of such staining introduces further factors which should be considered and controlled. Color analysis is a very complex problem with respect to reproducibly analyzing histological sections. The brightness component of the image should be independent of the colors within the image, in conventional RGB (red, green and blue) signalling mode this is not possible, while when using HSI (hue saturation and intensity) mode this becomes possible, and factors like staining intensity and brightness of the image become much more accountable and controllable. With these factors identified, we consider that the quantitative image analysis approach does allow comparison of patterns of bone-related mRNAs and demonstrates differences in expression in these osteogenic factors depending on distance from the prosthesis, tissue type, patient and device. © 1998 Kluwer Academic Publishers
Starting Page	691
Ending Page	694
Page Count	4
File Format	PDF
ISSN	09574530
Journal	Journal of Materials Science: Materials in Medicine
Volume Number	9
Issue Number	12
e-ISSN	15734838
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	1998-01-01
Publisher Place	Boston
Access Restriction	Subscribed
Subject Keyword	Biotechnology Polymer Sciences Characterization and Evaluation Materials
Content Type	Text
Resource Type	Article
Subject	Biomaterials Biophysics Bioengineering Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Factors affecting the static shear strength of the prosthetic stem–bone cement interface

Human biological reactions at the interface between bone tissue and polymethylmethacrylate cement

Fatigue of bone cement with simulated stem interface porosity

Quantification of bone ingrowth within bone-derived porous hydroxyapatite implants of varying density

X-ray diffraction of bone at the interface with hydroxyapatite-coated versus uncoated metal implants

Soluble phosphate glass fibres for repair of bone-ligament interface

Electron microscopic investigation on the osteogenesis at titanium implant/bone marrow interface under masticatory loading

Direct activation of mast cells by prosthetic biomaterial particles

Curing characteristics of acrylic bone cement

Quantification of the bone-related mRNAs at the bone/prosthetic interface

Similar Documents

Factors affecting the static shear strength of the prosthetic stem–bone cement interface

Human biological reactions at the interface between bone tissue and polymethylmethacrylate cement

Fatigue of bone cement with simulated stem interface porosity

Quantification of bone ingrowth within bone-derived porous hydroxyapatite implants of varying density

X-ray diffraction of bone at the interface with hydroxyapatite-coated versus uncoated metal implants

Soluble phosphate glass fibres for repair of bone-ligament interface

Electron microscopic investigation on the osteogenesis at titanium implant/bone marrow interface under masticatory loading

Direct activation of mast cells by prosthetic biomaterial particles

Curing characteristics of acrylic bone cement

Quantification of the bone-related mRNAs at the bone/prosthetic interface