NDLI: In vitro real-time characterization of cell attachment and spreading

Content Provider	Springer Nature Link
Author	Fredriksson, C. Khilman, S. Kasemo, B. Steel, D. M.
Copyright Year	1998
Abstract	A method based on the piezoelectric quartz crystal microbalance (QCM) technique for in vitro real-time characterization of cell attachment and spreading on surfaces has been developed. The method simultaneously measures the resonant frequency, f, and the dissipation energy, D, of the oscillating system. The QCM responses are sensitive to very small amounts (a few hundreds) of cells and highly specific to surface chemical properties. The first results from deposition of cells on two polystyrene surfaces of different wettability in serum-containing medium are reported. It has previously been shown that a decrease in f is related to the degree of cell spreading. In our data it appears that the extent or quality of cell attachment is reflected in an increase in D caused by adhering cells. The combined information from f and D measured by this technique might therefore be useful to probe cell–surface interactions for biomaterials. © 1998 Kluwer Academic Publishers
Starting Page	785
Ending Page	788
Page Count	4
File Format	PDF
ISSN	09574530
Journal	Journal of Materials Science: Materials in Medicine
Volume Number	9
Issue Number	12
e-ISSN	15734838
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	1998-01-01
Publisher Place	Boston
Access Restriction	Subscribed
Subject Keyword	Biotechnology Polymer Sciences Characterization and Evaluation Materials
Content Type	Text
Resource Type	Article
Subject	Biomaterials Biophysics Bioengineering Biomedical Engineering

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