NDLI: Exposure of the murine RAW 264.7 macrophage cell line to dicalcium silicate coating: assessment of cytotoxicity and pro-inflammatory effects

Content Provider	Springer Nature Link
Author	Chen, Liangjiao Zhang, Yanli Liu, Jia Wei, Limin Song, Bin Shao, Longquan
Copyright Year	2016
Abstract	Inflammatory effects are significant elements of the immune response to biomaterials. Previously, we reported inflammatory effects in response to dicalcium silicate (Ca$_{2}$SiO$_{4,}$ C$_{2}$S) particles. However, the immunological effects of C$_{2}$S coatings have not been studied. C$_{2}$S often used as coatings materials in orthopedic and dentistry applications. It may have different effect from C$_{2}$S particles. Further, it remains unclear whether C$_{2}$S coating is equally biocompatible as 45S5 coating. The aim of this study was to test the cytotoxicity and pro-inflammatory effects of C$_{2}$S coating on RAW 264.7 macrophages. C$_{2}$S and 45S5 coatings were characterized using scanning electron microscopy (SEM), atomic force microscopy (AFM), energy dispersive analysis (EDS) and X-ray diffraction (XRD). inductively coupled plasma optical emission spectroscopy (ICP-OES) was used to detect ionic concentrations after soaking coated discs in medium. The cytotoxicity of C$_{2}$S and 45S5 coatings against RAW 264.7 macrophages was measured using the LDH Cytotoxicity Assay Kit, Cell Counting Kit-8 (CCK-8) assays and flow cytometry for apoptosis assays. The gene and protein expression of TNF-α, IL-6 and IL-1β were detected using RT-q PCR and ELISA, respectively. The tested coating materials are not cytotoxic to macrophages. The C$_{2}$S-coated surface stimulated macrophages to express pro-inflammatory mediators, such as TNF-α, IL-6 and IL-1β, and C$_{2}$S coating caused less IL-6 but greater IL-1β production than the 45S5 coating. C$_{2}$S coating have no cytotoxicity when directly cultured with macrophages. C$_{2}$S and 45S5 coatings both have the potential to induce pro-inflammatory effects, and the biocompatibility of C$_{2}$S is similar to that of 45S5.
Starting Page	1
Ending Page	11
Page Count	11
File Format	PDF
ISSN	09574530
Journal	Journal of Materials Science: Materials in Medicine
Volume Number	27
Issue Number	3
e-ISSN	15734838
Language	English
Publisher	Springer US
Publisher Date	2016-01-22
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	Biomaterials Biomedical Engineering Regenerative Medicine/Tissue Engineering Polymer Sciences Ceramics, Glass, Composites, Natural Methods Surfaces and Interfaces, Thin Films
Content Type	Text
Resource Type	Article
Subject	Biomaterials Biophysics Bioengineering Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Macrophage-mediated osteogenesis activation in co-culture with osteoblast on calcium silicate cement

Cytotoxicity of modified glass ionomer cement on odontoblast cells

Apatite bone cement reinforced with calcium silicate fibers

Streptococcus mutans adherence and biofilm formation on experimental composites containing dicalcium phosphate dihydrate nanoparticles

Inflammatory cell response to ultra-thin amorphous and crystalline hydroxyapatite surfaces

Phosphatidylethanolamine biomimetic coating increases mesenchymal stem cell osteoblastogenesis

Effect of silicate incorporation on in vivo responses of α-tricalcium phosphate ceramics

Macrophage response to chitosan/poly-(γ-glutamic acid) nanoparticles carrying an anti-inflammatory drug

Exploiting the inflammatory response on biomaterials research and development

Exposure of the murine RAW 264.7 macrophage cell line to dicalcium silicate coating: assessment of cytotoxicity and pro-inflammatory effects

Similar Documents

Macrophage-mediated osteogenesis activation in co-culture with osteoblast on calcium silicate cement

Cytotoxicity of modified glass ionomer cement on odontoblast cells

Apatite bone cement reinforced with calcium silicate fibers

Streptococcus mutans adherence and biofilm formation on experimental composites containing dicalcium phosphate dihydrate nanoparticles

Inflammatory cell response to ultra-thin amorphous and crystalline hydroxyapatite surfaces

Phosphatidylethanolamine biomimetic coating increases mesenchymal stem cell osteoblastogenesis

Effect of silicate incorporation on in vivo responses of α-tricalcium phosphate ceramics

Macrophage response to chitosan/poly-(γ-glutamic acid) nanoparticles carrying an anti-inflammatory drug

Exploiting the inflammatory response on biomaterials research and development

Exposure of the murine RAW 264.7 macrophage cell line to dicalcium silicate coating: assessment of cytotoxicity and pro-inflammatory effects