NDLI: Polyethylene wear particles induce TLR 2 upregulation in the synovial layer of mice

Content Provider	Springer Nature Link
Author	Paulus, A. C. Frenzel, J. Ficklscherer, A. Roßbach, B. P. Melcher, C. Jansson, V. Utzschneider, S.
Copyright Year	2013
Abstract	A cellular and receptor mediated response to ultra-high-molecular-weight-polyethylene (UHMWPE) wear particles results in a release of proinflammatory cytokines and induces an inflammatory reaction causing osteolysis in total joint replacement. This investigation offers insight into the toll-like receptor (TLR) mediated activation by polyethylene wear particles in the synovial layer of mice. We hypothesized that, similar to recent in vitro results, UHMWPE particles lead to an upregulation of TLR 1 and 2 and TLR 4 in vivo in the synovial tissue of mice as well. Therefore, UHMWPE particles were generated in a common knee simulator according to the ISO standard, separated by acid digestion and determined by scanning electron microscopy. Endotoxin was removed using a method based on ultracentrifugation. A particle suspension (50 μl; 0.1 vol./vol.%) was injected into the left knee joint of female Balb/c mice (n = 8). In a control group, phosphate-buffered saline was injected into the left knee of Balb/c mice (n = 8). The mice were sacrificed after 7 days. Immunohistochemical staining was performed with TLR 1, 2 and 4 polyclonal antibodies for Balb/c mice and evaluated by light microscopy. The particle-stimulated group showed a thickened synovial layer, an increased cellular infiltration and a TLR 2-upregulation in the synovial layer compared to the control group. An increased expression of TLR 1 and TLR 4 could not be demonstrated. These results indicate a mainly TLR 2-induced inflammation to polyethylene wear debris in the synovial layer of mice.
Starting Page	507
Ending Page	513
Page Count	7
File Format	PDF
ISSN	09574530
Journal	Journal of Materials Science: Materials in Medicine
Volume Number	25
Issue Number	2
e-ISSN	15734838
Language	English
Publisher	Springer US
Publisher Date	2013-11-19
Publisher Place	Boston
Access Restriction	Subscribed
Subject Keyword	Biomaterials Polymer Sciences Ceramics, Glass, Composites, Natural Methods Metallic Materials Characterization and Evaluation of Materials Surfaces and Interfaces, Thin Films
Content Type	Text
Resource Type	Article
Subject	Biomaterials Biophysics Bioengineering Biomedical Engineering

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