NDLI: Adhesion and growth of vascular smooth muscle cells in cultures on bioactive RGD peptide-carrying polylactides

Content Provider	Springer Nature Link
Author	Bacakova, Lucie Filova, Elena Kubies, Dana Machova, Ludka Proks, Vladimir Maliva, Vesela Lisa, Vera Rypacek, Frantisek
Copyright Year	2007
Abstract	The surface of poly(l-lactide) (PLLA) films deposited on glass coverslips was modified with poly(dl-lactide) (PDLLA), or 1:4 mixtures of PDLLA and PDLLA-b-PEO block copolymers, in which either none, 5% or 20% of the copolymer molecules carried a synthetic extracellular matrix-derived ligand for integrin adhesion receptors, the GRGDSG oligopeptide, attached to the end of the PEO chain. The materials, perspective for vascular tissue engineering, were seeded with rat aortic smooth muscle cells (11,000 cells/cm$^{2}$) and the adhesion, spreading, DNA synthesis and proliferation of these cells was followed on inert and bioactive surfaces. In 24-h-old cultures in serum-supplemented media, the number of cells adhering to the PDLLA-b-PEO copolymer was almost eight times lower than that on the control PDLLA surface. On the surfaces containing 5% and 20% GRGDSG-PEO-b-PDLLA copolymer, the number of cells increased 6- and 3-fold respectively, compared to the PDLLA-b-PEO copolymer alone. On PDLLA-b-PEO copolymer alone, the cells were typically round and non-spread, whereas on GRGDSG-modified surfaces the cell spreading areas approached those found on PDLLA, reaching values of 991 μm$^{2}$ and 611 μm$^{2}$ for 5% and 20% GRGDSG respectively, compared to 958 μm$^{2}$ for PDLLA. The cells on GRGDSG-grafted copolymers were able to form vinculin-containing focal adhesion plaques, to synthesize DNA and even proliferate in a serum-free medium, which indicates specific binding to the GRGDSG sequences through their adhesion receptors.
Starting Page	1317
Ending Page	1323
Page Count	7
File Format	PDF
ISSN	09574530
Journal	Journal of Materials Science: Materials in Medicine
Volume Number	18
Issue Number	7
e-ISSN	15734838
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2007-03-27
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	Surfaces and Interfaces, Thin Films Ceramics, Glass, Composites, Natural Methods Metallic Materials Polymer Sciences Characterization and Evaluation of Materials Biomaterials
Content Type	Text
Resource Type	Article
Subject	Biomaterials Biophysics Bioengineering Biomedical Engineering

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