NDLI: Calcium ions promote osteogenic differentiation and mineralization of human dental pulp cells: implications for pulp capping materials

Content Provider	Springer Nature Link
Author	An, Shaofeng Gao, Yan Ling, Junqi Wei, Xi Xiao, Yin
Copyright Year	2011
Abstract	Calcium (Ca) is the main element of most pulp capping materials and plays an essential role in mineralization. Different pulp capping materials can release various concentrations of Ca ions leading to different clinical outcomes. The purpose of this study was to investigate the effects of various concentrations of Ca ions on the growth and osteogenic differentiation of human dental pulp cells (hDPCs). Different concentrations of Ca ions were added to growth culture medium and osteogenic inductive culture medium. A Cell Counting Kit-8 was used to determine the proliferation of hDPCs in growth culture medium. Osteogenic differentiation and mineralization were measured by alkaline phosphatase (ALP) assay, Alizarin red S/von kossa staining, Ca content quantitative assay. The selected osteogenic differentiation markers were investigated by quantitative real-time polymerase chain reaction (qRT-PCR). Within the range of 1.8–16.2 mM, increased concentrations of Ca ions had no effect on cell proliferation, but led to changes in osteogenic differentiation. It was noted that enhanced mineralized matrix nodule formation was found in higher Ca ions concentrations; however, ALP activity and gene expression were reduced. qRT-PCR results showed a trend towards down-regulated mRNA expression of type I collagen and Runx2 at elevated concentrations of Ca ions, whereas osteopontin and osteocalcin mRNA expression were significantly up-regulated. Ca ions content in the culture media can significantly influence the osteogenic properties of hDPCs, indicating the importance of optimizing Ca ions release from dental pulp capping materials in order to achieve desirable clinical outcomes.
Starting Page	789
Ending Page	795
Page Count	7
File Format	PDF
ISSN	09574530
Journal	Journal of Materials Science: Materials in Medicine
Volume Number	23
Issue Number	3
e-ISSN	15734838
Language	English
Publisher	Springer US
Publisher Date	2011-12-22
Publisher Place	Boston
Access Restriction	Subscribed
Subject Keyword	Biomaterials Metallic Materials Ceramics, Glass, Composites, Natural Methods Polymer Sciences Surfaces and Interfaces, Thin Films Characterization and Evaluation of Materials
Content Type	Text
Resource Type	Article
Subject	Biomaterials Biophysics Bioengineering Biomedical Engineering

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