NDLI: Twenty new microsatellite loci for population structure and parentage studies of Parnassius apollo nevadensis (Lepidoptera; Papilionidae)

Content Provider	Springer Nature Link
Author	Mira, Óscar Martínez, Juan G. Dawson, Deborah A. Tinaut, Alberto Sánchez Prieto, Cristina
Copyright Year	2014
Abstract	We characterized 20 new microsatellite loci isolated from Parnassius apollo nevadensis. The 20 loci displayed 2–12 alleles and expected heterozygosities varied from 0.22 to 0.89; the markers were amplified in nine multiplex reactions. The combined non-exclusion probabilities of this set are 0.001084 and 0.000005 for the first and second parents, respectively. This set of markers will thus enable other studies on reproductive strategies and population genetics in this endangered and previously understudied species. We show, for the first time, evidence of multiple paternity and that females may successfully copulate with more than one male despite the presence of mating plugs. Future studies on this mating behavior and population genetics of the species using the 20 loci here presented will be very important to define its conservation status and management measures.
Starting Page	771
Ending Page	779
Page Count	9
File Format	PDF
ISSN	1366638X
Journal	Journal of Insect Conservation
Volume Number	18
Issue Number	5
e-ISSN	15729753
Language	English
Publisher	Springer International Publishing
Publisher Date	2014-08-07
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Lepidoptera Microsatellite markers Parentage analysis Parnassius apollo Population structure Polyandry Entomology Conservation Biology/Ecology Biodiversity Animal Ecology Life Sciences
Content Type	Text
Resource Type	Article
Subject	Ecology Animal Science and Zoology Nature and Landscape Conservation Insect Science

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