NDLI: Cloning and characterization of a Rab11 homologue in Gracilariopsis lemaneiformis

Content Provider	Springer Nature Link
Author	Ren, Xueying Zhang, Xuecheng Mao, Yunxiang Sui, Zhenghong Xu, Di Zang, Xiaonan
Copyright Year	2008
Abstract	A small GTPase-encoding gene, homologous to Rab11s from animals, was cloned from the red alga Gracilariopsis lemaneiformis. The gene was isolated from libraries generated by suppression subtractive hybridization between female gametophytes and tetrasporophytes, designated as GlRab11, and found to be phase-relative, upregulated in female gametophytes. Two transcripts of GlRab11 were obtained by RACE (rapid amplification of cDNA ends). One was a mature mRNA and the other was an intermediate formed during mRNA processing. The former had 737 nucleotides with an open reading frame of 648 nucleotides encoding 214 amino acid residues, 83 nucleotides of 5′-UTR (untranslated-region) and 12 nucleotides of 3′-UTR. The amino acid sequence analysis of GlRab11 showed that it contained a Rab domain and a double cysteine motif in the C terminus. Southern blot analysis revealed that GlRab11 was probably a single copy gene. Two recombinant GlRab11 proteins were able to bind GTP in vitro. The identification of proteins involved in the phase development of G. lemaneiformis might shed some light on the still obscure molecular events during phase formation in red algae.
Starting Page	1103
Ending Page	1109
Page Count	7
File Format	PDF
ISSN	09218971
Journal	Journal of Applied Phycology
Volume Number	20
Issue Number	6
e-ISSN	15735176
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-04-16
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Female gametophyte Rab11 GTPase Small GTPase Tetrasporophyte Ecology Plant Physiology Freshwater & Marine Ecology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Aquatic Science

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