NDLI: Cloning and analysis of the galactose-1-phosphate uridylyltransferase (galt) gene of Gracilariopsis lemaneiformis (Rhodophyta) and correlation between gene expression and agar synthesis

Content Provider	Springer Nature Link
Author	Li, Min Sui, Zhenghong Kang, Kyoung Ho Zhang, Xuecheng Zhu, Ming Yan, Binlun
Copyright Year	2009
Abstract	We report cloning and characterization of the galactose-1-phosphate uridylyltransferase (galt) gene from the multi-cellular agar-producing marine red alga Gracilariopsis (Gp.) lemaneiformis. The Gp. lemaneiformis galt gene contains an ORF of 1086 nt encoding a polypeptide of 362 amino acids. The deduced amino acid sequence is highly similar to the GALT polypeptides of Gracilaria gracilis (89%), E. coli (50%), and Saccharomyces cerevisiae (51%). The galt gene of Gp. lemaneiformis appears to contain no intron sequences. Southern analysis indicated that the Gp. lemaneiformis genome contains a single copy of the galt gene. Quantitative PCR analysis was carried on algal strains with different agar contents in order to explore the relationship between galt expression level and agar synthesis and to provide a possible marker for agar content. Real-time PCR indicated that four of five strains with high-agar-content group had higher transcript levels of galt than all strains examined in the low-agar group; while one was below the highest 2$^{−ΔΔCT}$ value of low-agar-content group. Though the difference in gene expression level between the two groups was only marginally significant (P = 0.066), it remains possible that an increase in galt gene expression may contribute to higher agar content. However, low levels of expression did not consistently correlate with low agar content. We conclude that galt gene expression may be an important but not decisive factor in agar synthesis, and may be useful in screening for high-agar-content strains.
Starting Page	157
Ending Page	164
Page Count	8
File Format	PDF
ISSN	09218971
Journal	Journal of Applied Phycology
Volume Number	22
Issue Number	2
e-ISSN	15735176
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-04-30
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Gracilariopsis lemaneiformis Galactose-1-phosphate uridylyltransferase Gene Quantitive PCR Agar Ecology Plant Physiology Freshwater & Marine Ecology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Aquatic Science

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