NDLI: Medium pH and nitrate concentration effects on accumulation of triacylglycerol in two members of the chlorophyta

Content Provider	Springer Nature Link
Author	Gardner, Robert Peters, Patrizia Peyton, Brent Cooksey, Keith E.
Copyright Year	2010
Abstract	Algal-derived biodiesel is of particular interest because of several factors including: the potential for a near-carbon-neutral life cycle, the prospective ability for algae to capture carbon dioxide generated from coal, and algae’s high per acre yield potential. Our group and others have shown that in nitrogen limitation, and for a single species of Chlorella, a rise in culture medium pH yields triacylglycerol (TAG) accumulation. To solidify and expand on these triggers, the influence and interaction of pH and nitrogen concentration on lipid production was further investigated on Chlorophyceae Scenedesmus sp. and Coelastrella sp. Growth was monitored optically and TAG accumulation was monitored by Nile red fluorescence and confirmed by gas chromatography. Both organisms grew in all treatments and TAG accumulation was observed by two distinct conditions: high pH and nitrogen limitation. The Scenedesmus sp. was shown to grow and produce lipids to a larger degree in alkaliphilic conditions (pH >9) and was used to further investigate the interplay between TAG accumulation from high pH and/or nitrate depletion. Results given here indicate that TAG accumulation per cell, monitored by Nile red fluorescence, correlates with pH at the time of nitrate depletion.
Starting Page	1005
Ending Page	1016
Page Count	12
File Format	PDF
ISSN	09218971
Journal	Journal of Applied Phycology
Volume Number	23
Issue Number	6
e-ISSN	15735176
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-12-03
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Triacylglycerol (TAG) Fatty acid methyl ester (FAME) Nile red fluorescence Nile-red-specific fluorescence Chlorophyta Freshwater & Marine Ecology Plant Sciences Ecology Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Plant Science Aquatic Science

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