NDLI: Light respiration in Chlorella sorokiniana

Content Provider	Springer Nature Link
Author	Kliphuis, Anna M. J. Janssen, Marcel End, Evert J. Martens, Dirk E. Wijffels, René H.
Copyright Year	2010
Abstract	Respiration and photosynthesis are two important processes in microalgal growth that occur simultaneously in the light. To know the rates of both processes, at least one of them has to be measured. To be able to measure the rate of light respiration of Chlorella sorokiniana, the measurement of oxygen uptake must be fast, preferably in the order of minutes. We measured the immediate post-illumination respiratory O$_{2}$ uptake rate (OUR) in situ, using fiber-optic oxygen microsensors, and a small and simple extension of the cultivation system. This method enables rapid and frequent measurements without disturbing the cultivation and growth of the microalgae. Two batch experiments were performed with C. sorokiniana in a short light-path photobioreactor, and the OUR was measured at different time points. The net oxygen production rate (net OPR) was measured online. Adding the OUR and net OPR gives the gross oxygen production rate (gross OPR), which is a measure for the oxygen evolution by photosynthesis. The gross OPR was 35–40% higher than the net OPR for both experiments. The respiration rate is known to be related to the growth rate, and it is suggested that faster algal growth leads to a higher energy (ATP) requirement, and as such, respiratory activity increases. This hypothesis is supported by our results, as the specific OUR is highest in the beginning of the batch culture when the specific growth rate is highest. In addition, the specific OUR decreases toward the end of the experiments until it reaches a stable value of around 0.3 mmol O$_{2}$ h$^{−1}$ g$^{−1}$. This value for the specific OUR is equal to the maintenance requirement of C. sorokiniana as determined in an independent study of (Zijffers et al. 2010 (in press)). This suggests that respiration could fulfill the maintenance requirements of the microalgal cells.
Starting Page	935
Ending Page	947
Page Count	13
File Format	PDF
ISSN	09218971
Journal	Journal of Applied Phycology
Volume Number	23
Issue Number	6
e-ISSN	15735176
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-11-03
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microalgae Chlorella sorokiniana Respiration Maintenance Photosynthetic efficiency Short light-path photobioreactor Freshwater & Marine Ecology Plant Sciences Ecology Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Plant Science Aquatic Science

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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