NDLI: Studies on the factors affecting the growth and hemolytic activity of Anabaena variabilis

Content Provider	Springer Nature Link
Author	Wang, Wei Song, Xiu Kai Ru, Shao Guo
Copyright Year	2007
Abstract	The hemolytic activity of the cell-free culture supernatant of Anabaena variabilis OL S1 was investigated using the hemolysis of rabbit erythrocytes as an assay. The culture medium of A. variabilis started to exhibit hemolytic activity at the late exponential growth phase, and maximized at the stationary phase. The hemolytic toxin is heat-stable and can be extracted in dichloromethane. The hemolytic activities under different temperature, light intensity and pH showed a high correlation with the cell densities (r=0.965, 0.951, 0.865, respectively), and the optimum condition is 28~30°C, pH 7.5~8.0, light intensity 120 μmol photons m$^{−2}$s$^{−1}$. The addition of 10~20 μg mL$^{−1}$ chloramphenicol, an inhibitor of protein synthesis, exhibited no marked suppression on the hemolytic activity. The supplement of 1~20 μg mL$^{−1}$ glycerol increased the hemolytic activity significantly, suggesting that synthesis of hemolysin was dependent on carbohydrate and lipid metabolism. The spectrum of erythrocyte sensitivity to the hemolysin indicated that rabbit erythrocytes were more sensitive to the hemolysin than were rat and human erythrocytes. Goldfish and cat erythrocytes were, however, insensitive to the hemolytic toxin of A. variabilis.
Starting Page	365
Ending Page	371
Page Count	7
File Format	PDF
ISSN	09218971
Journal	Journal of Applied Phycology
Volume Number	19
Issue Number	4
e-ISSN	15735176
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2007-02-10
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Anabaena variabilis blue-green algae cyanobacteria growth hemolysis hemolytic toxin Ecology Hydrobiology Plant Sciences Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Plant Science Aquatic Science

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