NDLI: A rapid and cost-effective method of genomic DNA isolation from cyanobacterial culture, mat and soil suitable for genomic fingerprinting and community analysis

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A rapid and cost-effective method of genomic DNA isolation from cyanobacterial culture, mat and soil suitable for genomic fingerprinting and community analysis

Production of Cocconeis neothumensis (Bacillariophyceae) biomass in batch cultures and bioreactors for biotechnological applications: light and nutrient requirements

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A rapid and cost-effective method of genomic DNA isolation from cyanobacterial culture, mat and soil suitable for genomic fingerprinting and community analysis

Content Provider	Springer Nature Link
Author	Srivastava, A. K. Ara, A. Bhargava, P. Mishra, Y. Rai, S. P. Rai, L. C.
Copyright Year	2007
Abstract	This study presents a phenol and lysozyme free protocol for genomic DNA isolation of cyanobacteria from culture, mats and soil. For an efficient and pure DNA isolation from cyanobacteria having tough cell wall, extra steps of glass beading and Sepharose 4B purification were added. The modified method gave a higher yield of DNA than the phenol: chloroform extraction method. Four parameters selected for purity testing of the isolated DNA were: (i) restriction digestion with Hind III, (ii) randomly amplified polymorphic DNA-PCR of axenic culture of cyanobacteria to assess phylogenetic relatedness, (iii) denaturing gradient gel electrophoretic (DGGE) analysis of cyanobacterial mat and soil to ascertain the applicability of the isolated DNA for community analysis, and (iv) sequencing of partial 16S rDNA of Hapalosiphon intricatus BHULCR1, Anabaena doliolum LCR1, Anabaena oryzae LCR2, Aulosira fertilissima LCR4, and Tolypothrix tenuis LCR7 and BLAST analysis to confirm their cyanobacterial identity. Data generated from above analyses lead us to conclude that the modified method in question is rapid, cost effective, health and time conscious and promising for genetic fingerprinting and community analysis of cyanobacteria from diverse habitats.
Starting Page	373
Ending Page	382
Page Count	10
File Format	PDF
ISSN	09218971
Journal	Journal of Applied Phycology
Volume Number	19
Issue Number	4
e-ISSN	15735176
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2007-02-10
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	16S rDNA BLAST cyanobacteria denaturing gradient gel electrophoresis (DGGE) randomly amplified polymorphic DNA (RAPD) Ecology Hydrobiology Plant Sciences Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Plant Science Aquatic Science

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