NDLI: Molecular cloning and expression analysis of glutathione peroxidase and glutathione reductase from Gracilaria lemaneiformis under heat stress

Content Provider	Springer Nature Link
Author	Lu, Ning Ding, Yan Zang, Xiao Nan Zhang, Xue Cheng Chen, Hao Mu, Xiao Sheng
Copyright Year	2013
Abstract	Glutathione peroxidase (GPx) and glutathione reductase (GR) are key enzymes in the antioxidant defense systems of living organisms and protect organisms from environmental stresses. To investigate the mechanism of resistance to heat stress in the red alga Gracilaria lemaneiformis, the cDNA sequences encoding glutathione peroxidase and glutathione reductase of two strains (wild type and heat-tolerant cultivar 981) of G. lemaneiformis were successfully cloned using reverse transcription PCR and rapid amplification cDNA ends techniques. cDNA encoding GPx has 930 nucleotides with an open reading frame (ORF) of 750 nucleotides, encoding 249 amino acid residues. cDNA encoding GR was 1,572 nucleotides with a 1,416-nucleotide ORF encoding 471 amino acids. No introns existed in genomic DNA of GPx and GR of G. lemaneiformis. Southern blotting indicated that there was only one copy of GPx and GR in both strains. Under heat stress, bioactivity and mRNA transcription levels of both GPx and GR were upregulated in cultivar 981 compared to those in the wild type. These results indicate that both GPx and GR may play important roles in the ability of G. lemaneiformis to resist high temperatures.
Starting Page	1925
Ending Page	1931
Page Count	7
File Format	PDF
ISSN	09218971
Journal	Journal of Applied Phycology
Volume Number	25
Issue Number	6
e-ISSN	15735176
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-03-23
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Expression analysis Gene cloning Glutathione peroxidase Glutathione reductase Gracilaria lemaneiformis Rhodophyta Heat stress Plant Sciences Freshwater & Marine Ecology Plant Physiology Ecology
Content Type	Text
Resource Type	Article
Subject	Plant Science Aquatic Science

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