NDLI: Protective effect of a freshwater alga, Spirogyra sp., against lipid peroxidation in vivo zebrafish and purification of antioxidative compounds using preparative centrifugal partition chromatography

Content Provider	Springer Nature Link
Author	Lee, Ji Hyeok Han, Jong Won Ko, Ju Young Lee, Wonwoo Ahn, Ginnae Kim, Chul Young Kim, Gwang Hoon Jeon, You Jin
Copyright Year	2015
Abstract	A freshwater alga, Spirogyra sp., collected in shallow ponds in South Korea, was evaluated for its antilipid peroxidative effect against 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced in vivo zebrafish, and antioxidative compounds from the alga were efficiently identified using 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS$^{+}$) online high-performance liquid chromatography (HPLC) and preparative centrifugal partition chromatography. The ethyl acetate fraction of Spirogyra sp. (SPE) in each fraction showed the strongest 2,2-diphenyl-1-picrylhydrazyl scavenging activity and significantly scavenged 2′,7′-dichlorodihydrofluorescein diacetate and diphenyl-1-pyrenylphosphine fluorescence, respectively, in AAPH-induced zebrafish embryo without any cytotoxicity in the concentrations between 25 and 50 μg mL$^{−1}$. The two main antioxidative compounds in SPE were confirmed by ABTS$^{+}$ online HPLC and were identified as gallic acid and methyl gallate, respectively, by HPLC–diode array detection (DAD)–electrospray ionization (ESI)/mass spectrometry (MS), $^{1}$H- and $^{13}$C-NMR. We conclude therefore that Spirogyra sp. is rich in gallic acid and methyl gallate, and it might be useful as a strong antilipid peroxidation material.
Starting Page	181
Ending Page	189
Page Count	9
File Format	PDF
ISSN	09218971
Journal	Journal of Applied Phycology
Volume Number	28
Issue Number	1
e-ISSN	15735176
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-03-07
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Spirogyra sp. Antilipid peroxidation ABTS$^{+}$ online HPLC Centrifugal partition chromatography (CPC) Zebrafish Plant Sciences Freshwater & Marine Ecology Plant Physiology Ecology
Content Type	Text
Resource Type	Article
Subject	Plant Science Aquatic Science

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