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| Content Provider | Springer Nature Link |
|---|---|
| Author | Suh, Man Hee Pulakat, Lakshmidevi Gavini, Nara |
| Copyright Year | 2000 |
| Abstract | Several lines of experimental analyses on the ploidy status of Azotobacter vinelandii genome lead to the conclusion that it contains more than 40 copies of its chromosome and therefore it is a polyploid organism. The genetic evidence argues against the existence of polyploidy in these cells since the segregation pattern of genetic markers under lack of selection pressure mimic that of haploids. However, when A. vinelandii was made Nif− by inserting a kanamycin resistance marker gene in the nifDK sequence and the cells were selected for kanamycin resistance and Nif+ phenotype, we were able to score colonies that are both kanamycin resistant and Nif+. Therefore, when the cells were subjected to forced double selection of the same locus, they behaved as if they carried at least two chromosomes, one carrying the kanamycin resistance marker in the nifDK genes and the other carrying the intact nifDK genes. These analyses suggested that at least a diploidy status can be induced in these cells under selection pressure. |
| Starting Page | 101 |
| Ending Page | 107 |
| Page Count | 7 |
| File Format | |
| ISSN | 00166707 |
| Journal | Genetica |
| Volume Number | 110 |
| Issue Number | 2 |
| e-ISSN | 15736857 |
| Language | English |
| Publisher | Kluwer Academic Publishers |
| Publisher Date | 2000-01-01 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Plant Genetics & Genomics Human Genetics Animal Genetics and Genomics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Plant Science Medicine Animal Science and Zoology Insect Science |
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