NDLI: Development and validation of a novel, simple, and accurate spectrophotometric method for the determination of lead in human serum

Content Provider	Springer Nature Link
Author	Shayesteh, Tavakol Heidari Khajavi, Farzad Khosroshahi, Abolfazl Ghafuri Mahjub, Reza
Copyright Year	2015
Abstract	The determination of blood lead levels is the most useful indicator of the determination of the amount of lead that is absorbed by the human body. Various methods, like atomic absorption spectroscopy (AAS), have already been used for the detection of lead in biological fluid, but most of these methods are based on complicated, expensive, and highly instructed instruments. In this study, a simple and accurate spectroscopic method for the determination of lead has been developed and applied for the investigation of lead concentration in biological samples. In this study, a silica gel column was used to extract lead and eliminate interfering agents in human serum samples. The column was washed with deionized water. The pH was adjusted to the value of 8.2 using phosphate buffer, and then tartrate and cyanide solutions were added as masking agents. The lead content was extracted into the organic phase containing dithizone as a complexion reagent and the dithizone–Pb(II) complex was formed and approved by visible spectrophotometry at 538 nm. The recovery was found to be 84.6 %. In order to validate the method, a calibration curve involving the use of various concentration levels was calculated and proven to be linear in the range of 0.01–1.5 μg/ml, with an R 2 regression coefficient of 0.9968 by statistical analysis of linear model validation. The largest error % values were found to be −5.80 and +11.6 % for intra-day and inter-day measurements, respectively. The largest RSD % values were calculated to be 6.54 and 12.32 % for intra-day and inter-day measurements, respectively. Further, the limit of detection (LOD) was calculated to be 0.002 μg/ml. The developed method was applied to determine the lead content in the human serum of voluntary miners, and it has been proven that there is no statistically significant difference between the data provided from this novel method and the data obtained from previously studied AAS.
Starting Page	1
Ending Page	9
Page Count	9
File Format	PDF
ISSN	01676369
Journal	Environmental Monitoring and Assessment
Volume Number	188
Issue Number	1
e-ISSN	15732959
Language	English
Publisher	Springer International Publishing
Publisher Date	2015-12-02
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Lead Human serum Spectrophotometric detection Complexation Dithizone Environmental Monitoring/Analysis Environmental Management Ecotoxicology Atmospheric Protection/Air Quality Control/Air Pollution Ecology
Content Type	Text
Resource Type	Article
Subject	Pollution Medicine Management, Monitoring, Policy and Law

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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