NDLI: Development of a new technique to detect living cells of Xanthomonas campestris pv. campestris in crucifers seeds: the seed-qPCR

Content Provider	Springer Nature Link
Author	Laala, Samia Bouznad, Zouaoui Manceau, Charles
Copyright Year	2015
Abstract	Xanthomonas campestris pv. campestris is a seed-borne bacterium that causes black rot on Brassicaceae. Ensuring seed lot sanitary quality is the most efficient control strategy against bacterial diseases. Currently, the procedures adopted in the control of seed lots are mainly based on microbiological techniques combined with PCR or plant inoculation which is time and money consuming. The aim of this study was to propose a reliable and rapid detection technique of living X. c. pv. campestris in cabbage seeds. We have shown that not all cells of X. campestris is able to grown on rich medium after washing and soaking seeds as no colony of X. campestris was detected on inoculated seeds, whereas plantlets develop symptoms 7–14 days after germination. The PCR technique used alone does not address the viability of bacteria in samples. We set up a technique named seed-qPCR for the detection of living X. c. pv. campestris bacterial cells in seed lots. This technique is based on an enrichment of bacterial population associated with infected seeds by seed germination coupled with real-time Taq-man PCR after extraction of the target DNA. It is an inexpensive technique that allow the detection of down to 1 contaminated seed among 10 000 healthy seeds. The seed-qPCR method combines an efficient extraction based on bacterial multiplication on seedlings with a sensitive technique qPCR for the detection of bacteria in seed lots.
Starting Page	637
Ending Page	646
Page Count	10
File Format	PDF
ISSN	09291873
Journal	European Journal of Plant Pathology
Volume Number	141
Issue Number	4
e-ISSN	15738469
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-02-06
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Xanthomonas campestris Living bacteria Seed Detection Seed-qPCR Plant Pathology Plant Sciences Ecology Agriculture Life Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Horticulture Agronomy and Crop Science

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