NDLI: Effect of chemical stress imposed by Solanum nigrum in calreticulin and beta-1,4- endoglucanase genes and in infectivity of Pratylenchus goodeyi

Content Provider	Springer Nature Link
Author	Pestana, M. Abrantes, I. Gouveia, M.
Copyright Year	2014
Abstract	Isolation and expression of effector genes encoding proteins secreted by plant-parasitic nematodes into a host can be helpful in improving the understanding of parasitic interactions. In this study, calreticulin, a highly conserved Ca2+-binding and multifunctional protein, and beta-1,4-endoglucanase, a cell wall-degrading enzyme, both known to be secreted from oesophageal gland cells and injected through the nematode stylet into host tissue, were analysed. Full-length cDNAs from calreticulin (crt) and beta-1,4-endoglucanase (eng) with an estimated size of 1549 and 1342 bp, respectively, were isolated from the root-lesion nematode Pratylenchus goodeyi (Pg) by RT-PCR and RACE techniques. Pg-crt and Pg-eng cDNAs were characterized in silico, and their expression assessed by semi-quantitative PCR in nematodes exposed to a chemical stress provided by a Solanum nigrum extract showing nematicidal activity. It was demonstrated that the plant extract down-regulated the levels of Pg-crt mRNA, whereas the transcripts of Pg-eng mRNA held steady. This extract also affected nematode behaviour towards the roots since the number of nematodes that reached and penetrated the roots diminished when the exposure time rose. These observations indicate that the nematicidal compounds present in the plant extract were effective as a signal to influence the infection success of P. goodeyi in vitro and it might be tested against other phytoparasitic nematodes.
Starting Page	747
Ending Page	759
Page Count	13
File Format	PDF
ISSN	09291873
Journal	European Journal of Plant Pathology
Volume Number	141
Issue Number	4
e-ISSN	15738469
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-12-25
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Banana plant Beta-1,4-endoglucanase Calreticulin Chemical stress Gene expression Root-lesion nematode Solanum nigrum Plant Pathology Plant Sciences Ecology Agriculture Life Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Horticulture Agronomy and Crop Science

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