NDLI: Isolation and characterization of Panax ginseng geranylgeranyl-diphosphate synthase genes responding to drought stress

Content Provider	Springer Nature Link
Author	Rahimi, Shadi Kim, Yu Jin Devi, Balusamy Sri Renuka Khorolragchaa, Altanzul Sukweenadhi, Johan Yang, Deok Chun
Copyright Year	2015
Abstract	Geranylgeranyl-diphosphate synthases (GGDPS) catalyze branch point enzymatic reactions producing isoprenoid-derived products which are necessary for plant growth and responses to a wide range of biotic and abiotic stresses. In our study, full length geranylgeranyl-diphosphate synthase 1 (PgGGDPS1) and 2 (PgGGDPS2) cDNA were isolated and characterized from the flower of Panax ginseng and 4-year old P. ginseng cv. Gumpoong. The cDNA had open reading frame of 1032 and 1116 bp with a deduced amino acid sequence of 343 and 371 residues for GGDPS1 and GGDPS2, respectively. The calculated molecular mass of GGDPS1 and GGDPS2 were approximately 37.66 and 40.21 kDa with a predicated isoelectric point of 5.32 and 6.23 and predicted localization of plastid. A GenBank Blast X search revealed that the deduced amino acid of PgGGDPS1 shared a high degree of homology with GGDPS from Panax notoginseng. The transcription pattern of GGDPS genes was different at various developmental stages. Both GGDPS genes were highly expressed in aerial parts of the plant, especially in rapidly growing tissues such as 4-year old flower and stem tissues. Transcript level of PgGGDPS1 was differentially induced in ginseng not only during Pseudomonas syringae pv tomato infection but also after exposure to abiotic stresses. Our results suggested that the induction of GGDPS genes specifically PgGGDPS1 by drought stress may affect chlorophyll levels, intracellular GA content and accumulation of carotenoids as the precursor for higher production of ABA and possibly stomatal closure as the barrier for water loss.
Starting Page	747
Ending Page	758
Page Count	12
File Format	PDF
ISSN	09291873
Journal	European Journal of Plant Pathology
Volume Number	142
Issue Number	4
e-ISSN	15738469
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-04-14
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Drought Gene characterization Geranylgeranyl diphosphate synthase Organ Expression Panax ginseng Plant Pathology Plant Sciences Ecology Agriculture Life Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Horticulture Agronomy and Crop Science

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