NDLI: A CC-NBS-LRR type gene GHNTR1 confers resistance to southern root-knot nematode in Nicotiana.benthamiana and Nicotiana.tabacum

Content Provider	Springer Nature Link
Author	Zhang, Baolong Yang, Yuwen Wang, Jinyan Ling, Xitie Hu, Zhongze Liu, Tingli Chen, Tianzi Zhang, Wenhua
Copyright Year	2015
Abstract	Root-knot nematodes are obligate parasites that invade the roots of agricultural plants and induce the formation of specialized feeding structures, especially races 3 and 4 of the southern root-knot nematode. However, not much is known about the defense mechanisms of plants against the invasion of M. incognita race 1. In this study, we characterized and performed functional analysis of the CC-NBS-LRR domain gene, GHNTR1. Using the GHNTR1 promoter to drive the GUS marker gene, we found that GUS expression was high in the roots and shoots of seedlings, four leaves stages, and mature stages. When GHNTR1 was transiently expressed in Nicotiana. benthamiana and Nicotiana. tabacum, necrosis was observed in the leaves and detectable amounts of H2O2 had accumulated when compared to the control plants. Stable transformation of N. benthamiana with the GHNTR1 gene using Agrobacterium induced the expression of defense marker genes PR1, PR2, LOX, and ERF1. In addition, the transgenic N. benthamiana and N. tabacum plants exhibited higher resistance to M. incognita infection. When transgenic N. tabacum were challenged with M. incognita, callose deposition and peroxide accumulation were observed in the roots by aniline-blue and DAB staining, respectively. Sequencing and bioinformatic analysis of small RNA revealed 48 miRNAs were up-regulated and 32 miRNAs were down-regulated. Further, 345 genes were predicted as targets of miRNAs and these included genes that are involved in cell death, death, and stress response. Thus, GHNTR1 is the first gene isolated from cotton that confers resistance against M. incognita and it induced a series of hypersensitive responses in transgenic tobacco. These findings improve our understanding of the molecular mechanisms of the response of upland cotton to M. incognita infection.
Starting Page	715
Ending Page	729
Page Count	15
File Format	PDF
ISSN	09291873
Journal	European Journal of Plant Pathology
Volume Number	142
Issue Number	4
e-ISSN	15738469
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-04-03
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cotton GHNTR1 Hypersensitive response Tobacco Small RNA Plant Pathology Plant Sciences Ecology Agriculture Life Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Horticulture Agronomy and Crop Science

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