NDLI: Experience of the Latium region (Central Italy) as a pest-free area for monitoring of Xylella fastidiosa: distinctive features of molecular diagnostic methods

Content Provider	Springer Nature Link
Author	Modesti, V. Pucci, N. Lucchesi, S. Campus, L. Loreti, S.
Copyright Year	2016
Abstract	The presence of Xylella fastidiosa in the region of Apulia, Italy, represents one of the most serious plant-health emergencies of recent years that Italy and the entire European Union have had to deal with. The Italian Ministry of Agriculture transposed the European Union guidelines that define the necessary measures to prevent the introduction and spread of X. fastidiosa, and has organized a monitoring plan for preventive purposes across the entire national territory. In this study, we report the experience of the Latium region, Italy, as an example of a ‘pest-free’ area; 315 inspections were carried out for a total area of 416 ha. With the analysis mainly focused on asymptomatic plant material, the diagnosis was based on a molecular method characterized by high specificity and sensitivity: real-time PCR. This method allowed the presence of X. fastidiosa to be proved or excluded in the processed samples, even though visual examination occasionally revealed the presence of host plants with suspicious symptoms. An in-depth description of the adopted approaches is reported here, along with the advantages and disadvantages of this diagnostic method. New techniques, such as loop-mediated isothermal amplification of DNA (LAMP), can improve the procedures by maintaining high sensitivity and specificity, in view of the application of the analysis directly in the field.
Starting Page	557
Ending Page	566
Page Count	10
File Format	PDF
ISSN	09291873
Journal	European Journal of Plant Pathology
Volume Number	148
Issue Number	3
e-ISSN	15738469
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-12-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Xylella fastidiosa subsp. pauca CoDiRO strain Detection Real-time PCR Lamp Plant Pathology Plant Sciences Ecology Agriculture Life Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Horticulture Agronomy and Crop Science

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