NDLI: Efficient Agrobacterium tumefaciens-mediated target gene disruption in the maize pathogen Curvularia lunata

Content Provider	Springer Nature Link
Author	Zhang, Li Li, Hui Xiao, Shuqin Lu, Yuanyuan Li, Guofu Xue, Chunsheng Chen, Jie
Copyright Year	2015
Abstract	Curvularia lunata causes Curvularia leaf spot disease of maize, resulting in periodically significant yield losses around the world. To understand the molecular mechanisms of fungal pathogenicity and virulence factors contributed to the host, here we report a knockout transformation system against target genes. The concentration of conidia, Agrobacterium cell density, and method of co-incubation were optimized for deletion of the gene encoding 1, 3, 8-trihydroxynaphthalene reductase (Brn1), a gene in the melanin biosynthesis pathway, as a test case. Transformants contained a single T-DNA copy. The Brn1 mutant strain was reduced in virulence compared with the wild type strain when inoculated on susceptible maize. Transformation efficiency was 130 ± 10 transformants per 1 × 105 germlings and homologous recombination efficiency was 60.0 to 100.0 %.
Starting Page	155
Ending Page	165
Page Count	11
File Format	PDF
ISSN	09291873
Journal	European Journal of Plant Pathology
Volume Number	145
Issue Number	1
e-ISSN	15738469
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-12-16
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Curvularia lunata 1, 3, 8-trihydroxynaphthalene reductase (Brn1) Agrobacterium tumefaciens-mediated transformation Target gene knockout Plant Pathology Plant Sciences Ecology Agriculture Life Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Horticulture Agronomy and Crop Science

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