NDLI: Morphological and molecular characterization of sclerotium rolfsii associated with fruit rot of Cucurbita maxima

Content Provider	Springer Nature Link
Author	Mahadevakumar, S. Yadav, Vandana Tejaswini, G. S. Janardhana, G. R.
Copyright Year	2015
Abstract	In the present study, fruit rot disease of Cucurbita maxima observed in many crop fields of Southern Karnataka, India is reported. The fruit rot disease on Pumpkin appears as water soaked lesion on the surface of the fruit followed by the development of large rotten areas with abundant white mycelial growth. The fungal pathogen was isolated on potato dextrose agar (PDA) medium and development of whitish fast-growing mycelia with numerous reddish brown sclerotia was observed. The sclerotia were initially white and turned dark brown upon maturation. Genomic DNA was isolated and internal transcribed region of ribosomal DNA was amplified using ITS1-ITS4 universal primers. Based on cultural, morphological and molecular characteristics, the fungal pathogen was identified as Sclerotium rolfsii. The rDNA sequence results also showed 99–100 % similarity with reference sequence AB075298.1 and JF966208.1 confirming pathogen identity. The pathogenicity test conducted on healthy detached fruits produced fruit rot symptoms after 6–8 days of post inoculation. S. rolfsii is known to infect many economically important crop plants at various stages of its growth and development. To the best of our knowledge, this is the first report on the association of S. rolfsii with fruit rot of C. maxima in India.
Starting Page	215
Ending Page	219
Page Count	5
File Format	PDF
ISSN	09291873
Journal	European Journal of Plant Pathology
Volume Number	145
Issue Number	1
e-ISSN	15738469
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-11-19
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Sclerotium rolfsii Cucurbita maxima Fruit rot Pathogenicity Molecular identification Plant Pathology Plant Sciences Ecology Agriculture Life Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Horticulture Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Neofusicoccum parvum associated with fruit rot and shoot blight of peaches in Greece

Characterization and pathogenicity of Rhizoctonia spp. isolated from winter squash in the Black Sea region of Turkey

Identification and characterization of Diaporthe vaccinii Shear causing upright dieback and viscid rot of cranberry in Poland

Efficiency assessment of the antifungal metabolites from Sclerotium cepivorum against onion white rot disease

Identification and characterization of Fusarium spp. associated with root rots of field pea in North Dakota

Diagnosis and Integrated Management of Fruit Rot in Cucurbita argyrosperma, Caused by Sclerotium rolfsii

Characterization of Sclerotinia nivalis causing Sclerotinia rot of Pulsatilla koreana in China

Identification and characterization of Phytopythium helicoides causing stem rot of Shatangju mandarin seedlings in China

Inoculation with Sclerotium rolfsii, cause of stem rot in Jerusalem artichoke, under field conditions

Morphological and molecular characterization of sclerotium rolfsii associated with fruit rot of Cucurbita maxima

Similar Documents

Neofusicoccum parvum associated with fruit rot and shoot blight of peaches in Greece

Characterization and pathogenicity of Rhizoctonia spp. isolated from winter squash in the Black Sea region of Turkey

Identification and characterization of Diaporthe vaccinii Shear causing upright dieback and viscid rot of cranberry in Poland

Efficiency assessment of the antifungal metabolites from Sclerotium cepivorum against onion white rot disease

Identification and characterization of Fusarium spp. associated with root rots of field pea in North Dakota

Diagnosis and Integrated Management of Fruit Rot in Cucurbita argyrosperma, Caused by Sclerotium rolfsii

Characterization of Sclerotinia nivalis causing Sclerotinia rot of Pulsatilla koreana in China

Identification and characterization of Phytopythium helicoides causing stem rot of Shatangju mandarin seedlings in China

Inoculation with Sclerotium rolfsii, cause of stem rot in Jerusalem artichoke, under field conditions

Morphological and molecular characterization of sclerotium rolfsii associated with fruit rot of Cucurbita maxima