NDLI: Characteristic of Monilinia spp. fungi causing brown rot of pome and stone fruits in Poland

Content Provider	Springer Nature Link
Author	Poniatowska, Anna Michalecka, Monika Bielenin, Anna
Copyright Year	2012
Abstract	Brown rot, caused by fungi belonging to the genus Monilinia, is one of the most important diseases of stone and pome trees in the world. During the summers of 2010 and 2011, a total of 670 Monilinia spp. isolates were obtained from infected fruits. They were collected from different commercial stone and pome fruit orchards, located in northern, southern and central Poland. All isolates were identified using multiplex PCR. Twenty isolates obtained from plum, peach and apple fruits were identified as M. polystroma and 5 isolates from plums as M. fructicola. The remaining isolates were identified as M. fructigena or M. laxa. The identification of the isolates was also confirmed on the basis of growth characteristics in culture according to the EPPO standard PM 7/18. A comparison of morphological features of four Monilinia spp. growing on two selective growth media, APDA-F500 and CHA, indicated significant differences between these species. In artificial inoculation of fruits, all the examined Monilinia spp. isolates were pathogenic. The species affiliation of M. polystroma and M. fructicola isolates collected from orchards in Poland was confirmed on the base of phylogenetic and sequence analysis of the internal transcribed spacer (ITS1/5.8S rDNA/ITS2) region of ribosomal DNA.
Starting Page	855
Ending Page	865
Page Count	11
File Format	PDF
ISSN	09291873
Journal	European Journal of Plant Pathology
Volume Number	135
Issue Number	4
e-ISSN	15738469
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-11-29
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Stone and pome fruits PCR ITS sequencing Pathogenicity test Morphological characterization Plant Pathology Plant Sciences Ecology Agriculture Life Sciences
Content Type	Text
Resource Type	Book Review
Subject	Plant Science Horticulture Agronomy and Crop Science

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