NDLI: Biological control of peach brown rot (Monilinia spp.) by Bacillus subtilis CPA-8 is based on production of fengycin-like lipopeptides

Content Provider	Springer Nature Link
Author	Yánez Mendizábal, Viviana Zeriouh, Houda Viñas, Inmaculada Torres, Rosario Usall, Josep Vicente, Antonio Pérez García, Alejandro Teixidó, Neus
Copyright Year	2011
Abstract	Bacillus subtilis CPA-8, a strain with demonstrated ability to control Monilinia spp. in peaches, was studied to elucidate its mechanisms of antifungal activity. Growth inhibition assays using cell-free supernatants and butanolic extracts showed strong antifungal activities against Monilinia laxa and Monilinia fructicola. By comparison with the reference B. subtilis strains UMAF6614 and UMAF6639, fengycin, iturin and surfactin lipopeptides were identified by thin layer chromatography in butanolic extracts from cell-free supernatants, indicating that antibiosis could be a major factor involved in the biological control ability of CPA-8. TLC-bioautography analysis confirmed the presence of fengycin, iturin and surfactin lipopeptides but strong antifungal activity could be associated only with fengycin lipopeptides. These results were definitively supported by mutagenesis analysis targeted to suppress fengycin biosynthesis by disruption of the B. subtilis fenB gene. By TLC-bioautography analysis it was possible to identify transformants from CPA-8 with reduced or suppressed antifungal activity, and this phenotype was associated with the lack of fengycin bands. Fruit trials confirmed that fengycin-defective mutants and their cell-free supernatants lost their ability to control peach brown rot disease in comparison with CPA-8 wild type strain or Serenade Max®, a commercial formulation based on B. subtilis. Furthermore, population dynamics studies determined that CPA-8 fengycin-deficient mutants survived in wounds in peach fruit equally well as the CPA-8 wild type. Taken together our data indicate that fengycin-like lipopeptides play a major role in the biological control potential of B. subtilis CPA-8 against peach brown rot.
Starting Page	609
Ending Page	619
Page Count	11
File Format	PDF
ISSN	09291873
Journal	European Journal of Plant Pathology
Volume Number	132
Issue Number	4
e-ISSN	15738469
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-11-27
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Antibiosis Biocontrol Lipopeptides Monilinia laxa Monilinia fructicola Postharvest diseases Life Sciences Agriculture Plant Sciences Ecology Plant Pathology
Content Type	Text
Resource Type	Book Review
Subject	Plant Science Horticulture Agronomy and Crop Science

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Combination of hot water, Bacillus subtilis CPA-8 and sodium bicarbonate treatments to control postharvest brown rot on peaches and nectarines

Secondary inoculum dynamics of Monilinia spp. and relationship to the incidence of postharvest brown rot in peaches and the weather conditions during the growing season

A novel strategy to reduce overwintering inoculum of Monilinia laxa

Inhibition on brown rot disease and induction of defence response in harvested peach fruit by nitric oxide solution

Relationship between the incidence of latent infections caused by Monilinia spp. and the incidence of brown rot of peach fruit: factors affecting latent infection

Occurrence of Monilinia laxa and M. fructigena after introduction of M. fructicola in peach orchards in Spain

Conidial density of Monilinia spp. on peach fruit surfaces in relation to the incidences of latent infections and brown rot

Signals induced by exogenous nitric oxide and their role in controlling brown rot disease caused by Monilinia fructicola in postharvest peach fruit

Taxonomy, distribution, epidemiology, disease cycle and management of brown rot disease of peach (Monilinia spp.)

Biological control of peach brown rot (Monilinia spp.) by Bacillus subtilis CPA-8 is based on production of fengycin-like lipopeptides

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