NDLI: Can the use of medical muds cause genotoxicity in eukaryotic cells? A trial using comet assay

Content Provider	Springer Nature Link
Author	Gerencsér, Gellért Szendi, Katalin Berényi, Károly Varga, Csaba
Copyright Year	2014
Abstract	Despite the lack of knowledge of their exact effects, peloids (natural muds) are widely applied in clinical treatment and prevention of different diseases, especially in rheumatic and gynecological disorders or skin diseases. Primarily we have information on their inorganic components, but only limited data are available on the organic components and nothing on their mechanism of chemical action. The objective of the present study was to detect the DNA-damaging effects (possible genotoxic effect) of peloid samples using the single-cell comet assay on Long Evans rat lymphocytes, human lymphocytes, and Eisenia fetida coelomocytes. Rat and human lymphocytes were exposed to the in toto peloid samples, in vitro. The Eisenia cells were extracted from the coelom of animals kept in the intact peloid sample. An indicator derived from the DNA fluorescence intensity was used in the statistical evaluation. The predominantly organic (Hévíz) sample showed a significant alteration from the negative control in several cases, while the inorganic (Kolop) applied did not. A higher quantity of organic compounds may have an important role in the emergence of DNA damage. The results revealed that medical muds have not only positive health effects but can also contain substances with potential human toxicity risk. Our research provides essential steps towards the creation of a toxicity profile and the possible safe use of peloids as medicinal therapy.
Starting Page	63
Ending Page	70
Page Count	8
File Format	PDF
ISSN	02694042
Journal	Environmental Geochemistry and Health
Volume Number	37
Issue Number	1
e-ISSN	15732983
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-07-26
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Coelomocyte Comet assay Genotoxicology Lymphocytes Medicinal muds Environmental Health Geochemistry Terrestrial Pollution Soil Science & Conservation Environmental Chemistry Public Health
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Medicine Geochemistry and Petrology Environmental Engineering Water Science and Technology

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