NDLI: The zebrafish/tumor xenograft angiogenesis assay as a tool for screening anti-angiogenic miRNAs

Content Provider	Springer Nature Link
Author	Chiavacci, Elena Rizzo, Milena Pitto, Letizia Patella, Francesca Evangelista, Monica Mariani, Laura Rainaldi, Giuseppe
Copyright Year	2014
Abstract	The zebrafish/tumor xenograft angiogenesis assay is used to approach tumor angiogenesis, a pivotal step in cancer progression and target for anti-tumor therapies. Here, we evaluated whether the assay could allow the identification of microRNAs having an anti-angiogenic potential. For that, we transfected DU-145 prostate cancer cells with four microRNAs (miR-125a, miR-320, miR-487b, miR-492) responsive to both anti- and pro-angiogenic stimuli applied to human umbilical vein endothelial cells. After transfection, DU-145 cells were injected close to the developing subintestinal vessels of transgenic Tg(Kdrl:eGFP)s843 zebrafish embryos that express green fluorescent protein under the control of Kdrl promoter. At 72 h post-fertilization, we observed that green fluorescent protein–positive neo-vessels infiltrated the graft of DU-145 transfected with miR-125a, miR-320, and miR-487b. Vice versa, neo-vessel formation and tumor cell infiltration were inhibited when DU-145 cells transfected with miR-492 were used. These results indicated that the zebrafish/tumor xenograft assay was adequate to identify microRNAs able to suppress the release of angiogenic growth factors by angiogenic tumor cells.
Starting Page	969
Ending Page	975
Page Count	7
File Format	PDF
ISSN	09209069
Journal	Cytotechnology
Volume Number	67
Issue Number	6
e-ISSN	15730778
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-06-20
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	microRNAs Tumor angiogenesis Zebrafish/tumor xenografts Prostate tumor cells Biotechnology Biomedicine general Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Clinical Biochemistry Bioengineering Biomedical Engineering Biotechnology

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