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The zebrafish/tumor xenograft angiogenesis assay as a tool for screening anti-angiogenic miRNAs.
| Content Provider | Europe PMC |
|---|---|
| Author | Chiavacci, Elena Rizzo, Milena Pitto, Letizia Patella, Francesca Evangelista, Monica Mariani, Laura Rainaldi, Giuseppe |
| Abstract | The zebrafish/tumor xenograft angiogenesis assay is used to approach tumor angiogenesis, a pivotal step in cancer progression and target for anti-tumor therapies. Here, we evaluated whether the assay could allow the identification of microRNAs having an anti-angiogenic potential. For that, we transfected DU-145 prostate cancer cells with four microRNAs (miR-125a, miR-320, miR-487b, miR-492) responsive to both anti- and pro-angiogenic stimuli applied to human umbilical vein endothelial cells. After transfection, DU-145 cells were injected close to the developing subintestinal vessels of transgenic Tg(Kdrl:eGFP)s843 zebrafish embryos that express green fluorescent protein under the control of Kdrl promoter. At 72 h post-fertilization, we observed that green fluorescent protein–positive neo-vessels infiltrated the graft of DU-145 transfected with miR-125a, miR-320, and miR-487b. Vice versa, neo-vessel formation and tumor cell infiltration were inhibited when DU-145 cells transfected with miR-492 were used. These results indicated that the zebrafish/tumor xenograft assay was adequate to identify microRNAs able to suppress the release of angiogenic growth factors by angiogenic tumor cells. Electronic supplementary material The online version of this article (doi:10.1007/s10616-014-9735-y) contains supplementary material, which is available to authorized users. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC4628928&blobtype=pdf |
| ISSN | 09209069 |
| Volume Number | 67 |
| DOI | 10.1007/s10616-014-9735-y |
| PubMed Central reference number | PMC4628928 |
| Issue Number | 6 |
| PubMed reference number | 24947063 |
| Journal | Cytotechnology |
| e-ISSN | 15730778 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2014-06-20 |
| Publisher Place | Dordrecht |
| Access Restriction | Open |
| Rights License | © Springer Science+Business Media Dordrecht 2014 |
| Subject Keyword | microRNAs Tumor angiogenesis Zebrafish/tumor xenografts Prostate tumor cells |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Clinical Biochemistry Bioengineering Biomedical Engineering Biotechnology |
