NDLI: Development and characterization of ten novel microsatellite markers from olive ridley sea turtle (Lepidochelys olivacea)

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Conservation Genetics : Volume 9, Issue 3, June 2008

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Conservation Genetics : Volume 6

Conservation Genetics : Volume 5

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Development and characterization of ten novel microsatellite markers from olive ridley sea turtle (Lepidochelys olivacea)

Content Provider	Springer Nature Link
Author	Aggarwal, Ramesh K. Lalremruata, Albert Velavan, Thirumalaisamy P. Pavani Sowjanya, Ayyadevara Singh, Lalji
Copyright Year	2007
Abstract	Olive ridleys, one of the widely distributed marine turtle species has undergone declines in recent years due to multiple anthropogenic factors warranting conservation efforts for which assessment of genetic variability in existing populations become critical. Here we describe development of ten new microsatellite markers from a short sequence repeat-enriched partial genomic DNA library, which are found to be highly informative for genetic studies. Eight of these markers when tested on 83 olive ridley turtles revealed high allelic diversity (4–27 alleles per marker), and high observed and expected heterozygosity estimates that ranged from 0.29 to 0.82 and 0.62 to 0.94, respectively. Two microsatellites were monomorphic in the tested olive ridley samples, but were found to be informative/polymorphic when tested on related marine turtle species. More importantly, nine of the new markers showed robust cross-species amplifications in three related species Dermochelys coriacea, Chelonia mydas and Eretmochelys imbricata. Thus, this study describes ten new microsatellite markers and also demonstrates their potential as efficient genetic markers in studies related to parentage analysis, population structure, phylogeography and species relationships of olive ridleys and other marine turtle species.
Starting Page	981
Ending Page	984
Page Count	4
File Format	PDF
ISSN	15660621
Journal	Conservation Genetics
Volume Number	9
Issue Number	4
e-ISSN	15729737
Language	English
Publisher	Springer Netherlands
Publisher Date	2007-10-11
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Lepidochelys olivacea Microsatellite Conservation Olive ridley marine turtle Evolutionary Biology Plant Sciences Animal Anatomy / Morphology / Histology Human Genetics
Content Type	Text
Resource Type	Article
Subject	Genetics Ecology, Evolution, Behavior and Systematics

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