NDLI: Eight new polymorphic microsatellite DNA markers for Sakhalin taimen Parahucho perryi

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Eight new polymorphic microsatellite DNA markers for Sakhalin taimen Parahucho perryi

Content Provider	Springer Nature Link
Author	Kopun, Theodora Winkler, Kathrin A. Weiss, Steven
Copyright Year	2008
Abstract	We developed eight polymorphic microsatellite markers for the endangered salmonid Parahucho perryi from genomic libraries enriched for (GACA)n and (GATA)n repeat sequences. Emphasis was placed on developing highly polymorphic, perfect repeats that could be scored with confidence. Six tetra- and two di-nucleotide loci were screened in 49 individuals from two populations on Sakhalin Island, Russia. Allelic variation was high with eight to 23 alleles per locus and expected heterozygosity ranging from 0.48 to 0.93. These highly variable markers should prove useful in evaluating inbreeding, gene flow and population structure in Sakhalin taimen throughout its range.
Starting Page	1089
Ending Page	1091
Page Count	3
File Format	PDF
ISSN	15660621
Journal	Conservation Genetics
Volume Number	10
Issue Number	4
e-ISSN	15729737
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-10-18
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Salmonidae Sakhalin Island Tetra-nucleotide repeats Endangered species Plant Sciences Evolutionary Biology Conservation Biology/Ecology Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Ecology, Evolution, Behavior and Systematics

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