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| Content Provider | Springer Nature Link |
|---|---|
| Author | Dembinski, Jennifer L. Krauss, Stefan |
| Copyright Year | 2009 |
| Abstract | Evidence suggests that multiple tumors, including pancreatic adenocarcinoma, display heterogeneity in parameters that are critical for tumor formation, progression and metastasis. Understanding heterogeneity in solid tumors is increasingly providing a plethora of new diagnostic and therapeutic approaches. In this study, a particular focus was put on identifying a subpopulation of stem cell-like, slow cycling tumor cells in a pancreas adenocarcinoma cell lines. Using a label retention technique a subpopulation of slow cycling cells (DiI+/SCC) was identified and further evaluated in the BxPC-3 and Panc03.27 cell lines. These slowly cycling cells managed to retain the lipophilic labeling dye DiI, while the bulk of the cells (>94%) did not. The DiI+/SCC population, showed only a partial overlap with the CSC markers CD24+/CD44+, CD133+ and ALDH but they survived chemotherapeutic treatment, and were able to recreate the initial heterogeneous tumor cell population. DiI+/SCCs exhibited an increased invasive potential as compared with their non-label retaining, faster cycling cells (DiI−/FCC). They also had increased tumorigenic potential and morphological changes resembling cells that have undergone an epithelial to mesenchymal transition (EMT). Analysis of DiI+/SCC cells by real time PCR revealed a selective up-regulation of tell tale components of the Hedgehog/TGFβ pathways, as well as a down-regulation of EGFR, combined with a shift in crucial components implied in EMT. The presented findings offer an expanded mechanistic understanding that associates tumor initiating potential with cycling speed and EMT in pancreatic cancer cell lines. |
| Starting Page | 611 |
| Ending Page | 623 |
| Page Count | 13 |
| File Format | |
| ISSN | 02620898 |
| Journal | Clinical & Experimental Metastasis |
| Volume Number | 26 |
| Issue Number | 7 |
| e-ISSN | 15737276 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2009-05-07 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Cancer stem cell EMT Gli 1 Pancreatic adenocarcinoma Slow cycling Sonic hedgehog (Shh) Surgical Oncology Hematology Oncology Biomedicine general Cancer Research |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cancer Research Oncology |
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