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| Content Provider | Springer Nature Link |
|---|---|
| Author | Tlapakova, Tereza Krylov, Vladimir Macha, Jaroslav |
| Copyright Year | 2005 |
| Abstract | Two paralogous mitochondrial malate dehydrogenase 2 (Mdh2) genes of Xenopus laevis have been cloned and sequenced, revealing 95% identity. Fluorescence in-situ hybridization (FISH) combined with tyramide amplification discriminates both genes; Mdh2a was localized into chromosome q3 and Mdh2b into chromosome q8. One kb cDNA probes detect both genes with 85% accuracy. The remaining signals were on the paralogous counterpart. Introns interrupt coding sequences at the same nucleotide as defined for mouse. Restriction polymorphism has been detected in the first intron of Mdh2a, while the individual variability in intron 6 of Mdh2b gene is represented by an insertion of incomplete retrotransposon L1Xl. Rates of nucleotide substitutions indicate that both genes are under similar evolutionary constraints. X. laevis Mdh2 genes can be used as markers for physical mapping and linkage analysis. |
| Starting Page | 699 |
| Ending Page | 706 |
| Page Count | 8 |
| File Format | |
| ISSN | 09673849 |
| Journal | Chromosome Research |
| Volume Number | 13 |
| Issue Number | 7 |
| e-ISSN | 15736849 |
| Language | English |
| Publisher | Kluwer Academic Publishers |
| Publisher Date | 2005-10-24 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | fluorescence in-situ hybridization (FISH) intron variability mitochondrial malate dehydrogenase 2 (Mdh2) retrotransposon L1Xl tyramide amplification Xenopus laevis Human Genetics Cell Biology Plant Genetics & Genomics Animal Genetics and Genomics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics |
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