NDLI: RETRACTED ARTICLE: Therapeutic DNA Vaccination as a Repair Strategy Following Spinal Cord Injury

Content Provider	Springer Nature Link
Author	Kou, Sheng Bin Xu, Gang Jiang, Xiao Dan Xu, Ru Xiang Tang, Yan Ping Xu, Gang Cai, Ying Qian Du, Mou Xuan Xiao, Zhi Cheng
Copyright Year	2009
Abstract	Myelin-derived proteins, such as tenascin-R (TN-R), myelin associate glycoprotein (MAG), oligodendrocyte-myelin glycoprotein (OMgp), and Nogo-A, inhibit the central nervous system regeneration. In this study, the DNA vaccine encoding for oligodendrocyte and myelin-related antigens was employed to attenuate the axonal growth inhibitory properties of myelin in the setting of spinal cord injury. Using a rat spinal cord dorsal hemisection model, the vaccine directed against the inhibitory epitopes of Nogo-A, MAG, OMgp, and TN-R was administered intramuscularly once a week following spinal cord injury, supplemented with local application of specific anti-sera against the four antigens. Anterograde labeling of dorsal column fibers showed active axonal regeneration through the lesion site at the eighth week following the treatment in experimental group but not in control groups. Light microscopic and ultrastructural analysis revealed that vaccination with these myelin-related antigens did not lead to demyelinating disease. OMgp and TN-R levels were down-regulated at the lesion site together with a parallel increase in growth-associated protein 43 levels in the treatment groups. This study reveals the effective approach of a DNA vaccine strategy by attaining the special antibody to direct neutralization of the myelin inhibitors during spinal cord injury.
Starting Page	275
Ending Page	282
Page Count	8
File Format	PDF
ISSN	02724340
Journal	Cellular and Molecular Neurobiology
Volume Number	30
Issue Number	2
e-ISSN	15736830
Language	English
Publisher	Springer US
Publisher Date	2009-09-16
Publisher Place	Boston
Access Restriction	Subscribed
Subject Keyword	DNA vaccine Spinal cord injury Axonal regeneration Myelin-associated protein (MAG) Tenascin-R (TN-R) Oligodendrocyte-myelin glycoprotein (OMgp) Nogo-A Animal Anatomy / Morphology / Histology Neurosciences
Content Type	Text
Subject	Cell Biology Medicine Cellular and Molecular Neuroscience

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