NDLI: Contribution of Intracellular Ca2+ Concentration and Protein Dephosphorylation to the Induction of Dopamine Release from PC12 cells by the Green Odor Compound Hexanal

Content Provider	Springer Nature Link
Author	Kobayashi, Yoko Kako, Hironari Yokogoshi, Hidehiko
Copyright Year	2009
Abstract	n-Hexanal (hexanal), a straight-chain six-carbon aldehyde, is mainly present in plants. Hexanal strongly affects the release of dopamine from rat striatal slices and rat pheochromocytoma (PC12) cells. In this study, we attempted to clarify the mechanism underlying the regulation of dopamine release by hexanal by using PC12 cells, which have the ability to synthesize, store, and release dopamine. The stimulation of PC12 cells with hexanal enhanced dopamine release in a time- and dose-dependent manner. Dopamine release was partially inhibited by pretreatment of the cells with BAPTA-AM, a cell-permeable Ca2+ chelator. In addition, the intracellular Ca2+ concentration was found to slowly increase after hexanal stimulation. Furthermore, the Src tyrosine kinase inhibitor PP2 partially inhibited hexanal-induced dopamine release. However, the levels of phosphorylated proteins decreased after hexanal stimulation. Hexanal stimulated the release of only a small amount of dopamine from reserpine-treated PC12 cells, in which the vesicular dopamine was depleted. These findings suggest that both an increase in the intracellular Ca2+ concentration and the dephosphorylation of phosphorylated proteins might be required for hexanal-stimulated release of dopamine, and that the dopamine released because of hexanal stimulation mainly comes from the dopamine vesicles. This study showed the cellular events that occurred in PC12 cells after stimulation of hexanal. Furthermore, it is important to examine the relationship between the cellular functions and the physiological effects of hexanal on dopamine release.
Starting Page	173
Ending Page	184
Page Count	12
File Format	PDF
ISSN	02724340
Journal	Cellular and Molecular Neurobiology
Volume Number	30
Issue Number	2
e-ISSN	15736830
Language	English
Publisher	Springer US
Publisher Date	2009-08-20
Publisher Place	Boston
Access Restriction	Subscribed
Subject Keyword	HPLC analysis Western blotting PP2 BAPTA-AM Reserpine Animal Anatomy / Morphology / Histology Neurosciences
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Cellular and Molecular Neuroscience

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Exocytotic Release of [3H]-Acetylcholine by Ouabain Involves Intracellular Ca2+ Stores in Rat Brain Cortical Slices

Dopamine-Induced Apoptosis Is Inhibited in PC12 Cells Expressing Bcl-2

Potentiation by adenosine of ATP-evoked dopamine release via a pertussis toxin-sensitive mechanism in rat phaeochromocytoma PC12 cells

Depolarization-induced changes of free intracellular Ca2+ concentration and of [3H]dopamine release in undifferentiated and differentiated PC12 cells

The Voltage-Gated Ca2+ Channel Is the Ca2+ Sensor of Fast Neurotransmitter Release

Dopamine Release Evoked by Beta Scorpion Toxin, Tityus Gamma, in Prefrontal Cortical Slices is Mediated by Intracellular Calcium Stores

Protective Effects of Paeoniflorin Against Glutamate-Induced Neurotoxicity in PC12 Cells via Antioxidant Mechanisms and Ca2+ Antagonism

Halothane Increases Non-vesicular [3H]dopamine Release from Brain Cortical Slices

Endogenous Dopamine Maintains Synchronous Oscillation of Intracellular Calcium in Primary Cultured-Mouse Midbrain Neurons

Contribution of Intracellular Ca2+ Concentration and Protein Dephosphorylation to the Induction of Dopamine Release from PC12 cells by the Green Odor Compound Hexanal

Similar Documents

Exocytotic Release of [3H]-Acetylcholine by Ouabain Involves Intracellular Ca2+ Stores in Rat Brain Cortical Slices

Dopamine-Induced Apoptosis Is Inhibited in PC12 Cells Expressing Bcl-2

Potentiation by adenosine of ATP-evoked dopamine release via a pertussis toxin-sensitive mechanism in rat phaeochromocytoma PC12 cells

Depolarization-induced changes of free intracellular Ca2+ concentration and of [3H]dopamine release in undifferentiated and differentiated PC12 cells

The Voltage-Gated Ca2+ Channel Is the Ca2+ Sensor of Fast Neurotransmitter Release

Dopamine Release Evoked by Beta Scorpion Toxin, Tityus Gamma, in Prefrontal Cortical Slices is Mediated by Intracellular Calcium Stores

Protective Effects of Paeoniflorin Against Glutamate-Induced Neurotoxicity in PC12 Cells via Antioxidant Mechanisms and Ca2+ Antagonism

Halothane Increases Non-vesicular [3H]dopamine Release from Brain Cortical Slices

Endogenous Dopamine Maintains Synchronous Oscillation of Intracellular Calcium in Primary Cultured-Mouse Midbrain Neurons

Contribution of Intracellular Ca2+ Concentration and Protein Dephosphorylation to the Induction of Dopamine Release from PC12 cells by the Green Odor Compound Hexanal