NDLI: Astaxanthin prevents in vitro auto-oxidative injury in human lymphocytes

Content Provider	Springer Nature Link
Author	Bolin, Anaysa P. Macedo, Rita C. Marin, Douglas P. Barros, Marcelo P. Otton, Rosemari
Copyright Year	2010
Abstract	Upon mitogen sensitization, lymphocytes undergo proliferation by oxyradical-based mechanisms. Through continuous resting–restimulation cycles, lymphocytes accumulate auto-induced oxidative lesions which lead to cell dysfunction and limit their viability. Astaxanthin (ASTA) is a nutritional carotenoid that shows notable antioxidant properties. This study aims to evaluate whether the in vitro ASTA treatment can limit oxyradical production and auto-oxidative injury in human lymphocytes. Activated lymphocytes treated with 5 µM ASTA showed immediate lower rates of O2•−/H2O2 production whilst NO• and intracellular Ca2+ levels were concomitantly enhanced (≤4 h). In long-term treatments (>24 h), the cytotoxicity test for ASTA showed a sigmoidal dose–response curve (LC50 = 11.67 ± 0.42 µM), whereas higher activities of superoxide dismutase and catalase in 5 µM ASTA-treated lymphocytes were associated to significant lower indexes of oxidative injury. On the other hand, lower proliferative scores of ASTA lymphocytes might be a result of diminished intracellular levels of pivotal redox signaling molecules, such as H2O2. Further studies are necessary to establish the ASTA-dose compensation point between minimizing oxidative damages and allowing efficient redox-mediated immune functions, such as proliferation, adhesion, and oxidative burst.
Starting Page	457
Ending Page	467
Page Count	11
File Format	PDF
ISSN	07422091
Journal	Cell Biology and Toxicology
Volume Number	26
Issue Number	5
e-ISSN	15736822
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-03-14
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Antioxidant Apoptosis Astaxanthin Carotenoid Lymphocyte Oxidative stress Biochemistry Pharmacology/Toxicology Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Health, Toxicology and Mutagenesis Toxicology

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