NDLI: Biochemical properties of encapsulated high-density 3-D HepG2 aggregates formed in an ultrasound trap for application in hepatotoxicity studies : Biochemical responses of encapsulated 3-D HepG2 aggregates

Please wait, while we are loading the content...

Selection for methotrexate resistance in mammalian cells bearing a Drosophila dihydrofolate reductase transgene : Methotrexate resistance in transgenic mammalian cells

Biochemical properties of encapsulated high-density 3-D HepG2 aggregates formed in an ultrasound trap for application in hepatotoxicity studies : Biochemical responses of encapsulated 3-D HepG2 aggregates

Induction of metallothionein in mouse cerebellum and cerebrum with low-dose thimerosal injection

C-reactive protein, sodium azide, and endothelial connexin43 gap junctions

Morphological alterations induced by boric acid and fipronil in the midgut of worker honeybee (Apis mellifera L.) larvae : Morphological alterations in the midgut of A. mellifera

Cell Biology and Toxicology : Volume 26, Issue 1, February 2010

Cell Biology and Toxicology : Volume 25

Cell Biology and Toxicology : Volume 24

Cell Biology and Toxicology : Volume 23

Cell Biology and Toxicology : Volume 22

Cell Biology and Toxicology : Volume 21

Cell Biology and Toxicology : Volume 20

Cell Biology and Toxicology : Volume 19

Cell Biology and Toxicology : Volume 18

Cell Biology and Toxicology : Volume 17

Cell Biology and Toxicology : Volume 16

Cell Biology and Toxicology : Volume 15

Cell Biology and Toxicology : Volume 14

Cell Biology and Toxicology : Volume 13

Biochemical properties of encapsulated high-density 3-D HepG2 aggregates formed in an ultrasound trap for application in hepatotoxicity studies : Biochemical responses of encapsulated 3-D HepG2 aggregates

Content Provider	Springer Nature Link
Author	Bazou, Despina
Copyright Year	2009
Abstract	This paper describes the alginate encapsulation of preformed high-density 3-D HepG2 cell aggregates that guarantees good maintenance of liver-specific biomarker expression. The process involves forming a high-density (≥7 × 104 cells/aggregate) discoid 3-D cell aggregate in an ultrasound trap, which is subsequently recovered and encapsulated in alginate/CaCl2 hydrogel. Glucose secretion/consumption, lactate release, detoxifying enzyme capacity, cytokeratin-18 expression as well as hypoxia were characterized in encapsulated 3-D HepG2 aggregates over 10 days in culture. Encapsulated 3-D HepG2 aggregates released glucose into the media, although this ability was exhibited only after 1 day in culture and was subsequently lost over the ensuing 9 days. In contrast, lactate was constantly released into the media. Significantly more lactate was secreted after 3 days in culture indicating a more hypoxic environment and hence a higher rate of anaerobic glycolysis. Aggregates consistently expressed cytokeratin-18. Cytochrome P450-1A1 activity reached a maximum on day 1 of culture followed by a progressive reduction to basal levels, while P450-3A4 activity was up-regulated in a time-dependent manner reaching a peak on day 7 in culture. Glutathione-S-transferase activity, on the other hand, was at more physiological levels and remained constant over the 10-day culture period. The ultrasound trap allowed the rapid (within 5 min) generation of uniformly shaped and sized aggregates. The results reported here suggest that ultrasound-formed 3-D HepG2 aggregates can serve as alternative in vitro models providing a quick outlook on toxicity, in a tissue-mimetic manner, thus offering the future option of a cost-effective screening platform for pharmaceutical development.
Starting Page	127
Ending Page	141
Page Count	15
File Format	PDF
ISSN	07422091
Journal	Cell Biology and Toxicology
Volume Number	26
Issue Number	2
e-ISSN	15736822
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-03-21
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Drug metabolism Cytokeratin-18 Encapsulation Glucose Hypoxia Ultrasound trap Biochemistry Pharmacology/Toxicology Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Health, Toxicology and Mutagenesis Toxicology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Development of an in vitro assay for the investigation of metabolism-induced drug hepatotoxicity

Chemically induced hepatotoxicity in human stem cell-induced hepatocytes compared with primary hepatocytes and HepG2

The application of cytokeratin-18 as a biomarker for drug-induced liver injury.

Cell Adhesion Dynamics and Actin Cytoskeleton Reorganization in HepG2 Cell Aggregates

Metabolomics-based strategy to assess drug hepatotoxicity and uncover the mechanisms of hepatotoxicity involved.

PCB 126 perturbs hypoxia-induced HIF-1 activity and glucose consumption in human HepG2 cells.

Allopurinol and 5-aminosalicylic acid influence thiopurine-induced hepatotoxicity in vitro

Hepatotoxicity due to mitochondrial dysfunction

Role of undecan-2-one on ethanol-induced apoptosis in HepG2 cells

Biochemical properties of encapsulated high-density 3-D HepG2 aggregates formed in an ultrasound trap for application in hepatotoxicity studies : Biochemical responses of encapsulated 3-D HepG2 aggregates

Similar Documents

Development of an in vitro assay for the investigation of metabolism-induced drug hepatotoxicity

Chemically induced hepatotoxicity in human stem cell-induced hepatocytes compared with primary hepatocytes and HepG2

The application of cytokeratin-18 as a biomarker for drug-induced liver injury.

Cell Adhesion Dynamics and Actin Cytoskeleton Reorganization in HepG2 Cell Aggregates

Metabolomics-based strategy to assess drug hepatotoxicity and uncover the mechanisms of hepatotoxicity involved.

PCB 126 perturbs hypoxia-induced HIF-1 activity and glucose consumption in human HepG2 cells.

Allopurinol and 5-aminosalicylic acid influence thiopurine-induced hepatotoxicity in vitro

Hepatotoxicity due to mitochondrial dysfunction

Role of undecan-2-one on ethanol-induced apoptosis in HepG2 cells

Biochemical properties of encapsulated high-density 3-D HepG2 aggregates formed in an ultrasound trap for application in hepatotoxicity studies : Biochemical responses of encapsulated 3-D HepG2 aggregates