NDLI: A circadian clock regulates sensitivity to cadmium in Paramecium tetraurelia

Content Provider	Springer Nature Link
Author	Hinrichsen, Robert D. Tran, Joseph R.
Copyright Year	2010
Abstract	The heavy metal cadmium is a dangerous environmental toxicant that can be lethal to humans and other organisms. This paper demonstrates that cadmium is lethal to the ciliated protozoan Paramecium tetraurelia and that a circadian clock modulates the sensitivity of the cells to cadmium. Various concentrations of cadmium were shown to increase the number of behavioral responses, decrease the swimming speed of cells, and generate large vacuole formation in cells prior to death. Cells were grown in either 12-h light/12-h dark or constant dark conditions exhibited a toxic response to 500 μM CdCl2; the sensitivity of the response was found to vary with a 24-h periodicity. Cells were most sensitive to cadmium at circadian time 0 (CT0), while they were least sensitive in the early evening (CT12). This rhythm persisted even when the cells were grown in constant dark. The oscillation in cadmium sensitivity was shown to be temperature-compensated; cells grown at 18°C and 28°C had a similar 24-h oscillation. Finally, phase shifting experiments demonstrated a phase-dependent response to light. These data establish the criteria required for a circadian clock and demonstrate that P. tetraurelia possesses a circadian-influenced regulatory component of the cadmium toxic response. The Paramecium system is shown to be an excellent model system for the study of the effects of biological rhythms on heavy metal toxicity.
Starting Page	379
Ending Page	389
Page Count	11
File Format	PDF
ISSN	07422091
Journal	Cell Biology and Toxicology
Volume Number	26
Issue Number	4
e-ISSN	15736822
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-01-27
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Cadmium Circadian rhythms Paramecium Biochemistry Pharmacology/Toxicology Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Health, Toxicology and Mutagenesis Toxicology

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