NDLI: Improvement of human dendritic cell culture for immunotoxicological investigations

Content Provider	Springer Nature Link
Author	Hymery, N. Sibiril, Y. Parent Massin, D.
Copyright Year	2006
Abstract	A toxic injury such as a decrease in the number of immature dendritic cells caused by a cytotoxic effect or a disturbance in their maturation process can be responsible for immunodepression. There is a need to improve in vitro assays on human dendritic cells used to detect and evaluate adverse effects of xenobiotics. Two aspects were explored in this work: cytotoxic effects of xenobiotics on immature dendritic cells, and the interference of xenobiotics with dendritic cell maturation. Dendritic cells of two different origins were tested. Dendritic cells obtained either from umbilical cord blood CD34+ cells or, for the first time, from umbilical cord blood monocytes. The cytotoxicity assay on immature dendritic cells has been improved. For the study of the potential adverse effects of xenobiotics on the maturation process of dendritic cells, several parameters were selected such as expression of markers (CD86, CD83, HLA-DR), secretion of interleukins 10 and 12, and proliferation of autologous lymphocytes. The relevance and the efficiency of the protocol applied were tested using two mycotoxins, T-2 toxin and deoxynivalence, DON, which are known to be immunosuppressive, and one phycotoxin, domoic acid, which is known not to have any immunotoxic effect. Assays using umbilical cord monocyte dendritic cell cultures with the protocol defined in this work, which involves a cytotoxicity study followed by evaluation of several markers of adverse effects on the dendritic cell maturation process, revealed their usefulness for investigating xenobiotic immunotoxicity toward immune primary reactions.
Starting Page	243
Ending Page	255
Page Count	13
File Format	PDF
ISSN	07422091
Journal	Cell Biology and Toxicology
Volume Number	22
Issue Number	4
e-ISSN	15736822
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2006-05-15
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	cytotoxicity dendritic cell maturation trichothecenes Pharmacology/Toxicology Biochemistry Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Health, Toxicology and Mutagenesis Toxicology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Progressive maturation resistance to microcystin-LR cytotoxicity in two different hepatospheroidal models

Transdentinal diffusion and cytotoxicity of self-etching adhesive systems

Spermine cytotoxicity to human colon carcinoma-derived cells (CaCo-2)

Metabolizing systems in cell culture cytotoxicity tests

Cell Culture Models for Neurotoxicology

Cell Culture Models for Hepatotoxicology

An improved resazurin-based cytotoxicity assay for hepatic cells

Mechanism of immunotoxicological effects of tributyltin chloride on murine thymocytes

Comparative particle-induced cytotoxicity toward macrophages and fibroblasts

Improvement of human dendritic cell culture for immunotoxicological investigations

Similar Documents

Progressive maturation resistance to microcystin-LR cytotoxicity in two different hepatospheroidal models

Transdentinal diffusion and cytotoxicity of self-etching adhesive systems

Spermine cytotoxicity to human colon carcinoma-derived cells (CaCo-2)

Metabolizing systems in cell culture cytotoxicity tests

Cell Culture Models for Neurotoxicology

Cell Culture Models for Hepatotoxicology

An improved resazurin-based cytotoxicity assay for hepatic cells

Mechanism of immunotoxicological effects of tributyltin chloride on murine thymocytes

Comparative particle-induced cytotoxicity toward macrophages and fibroblasts

Improvement of human dendritic cell culture for immunotoxicological investigations