NDLI: Metabolizing systems in cell culture cytotoxicity tests

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Metabolizing systems in cell culture cytotoxicity tests

Content Provider	Scilit
Author	Benford, Diane J. Reavy, Helen J. Hubbard, Susan A.
Copyright Year	1988
Description	Journal: Xenobiotica 1. The addition of 9000 g supernatant of rat liver homogenate (S9) or rat liver microsomal fractions to a cytotoxicity test system using BCL-D1 cells has been investigated. 2. The choice of culture medium influenced the intrinsic cytotoxicity of the metabolising system to the BCL-D1 cells. Use of Ham's F10 nutrient mixture resulted in greater cytotoxicity compared with several other media. 3. Microsomal fractions provided greater cytochrome P-450 dependent activation of cyclophosphamide and were less cytotoxic than S9. 4. Direct-acting toxic compounds, such as p-aminophenol, were less toxic in the presence of a metabolising system. This was due to protein-binding rather than enzymic detoxification.
Related Links	http://www.tandfonline.com/doi/pdf/10.3109/00498258809041703
Ending Page	656
Page Count	8
Starting Page	649
ISSN	00498254
e-ISSN	13665928
DOI	10.3109/00498258809041703
Journal	Xenobiotica
Issue Number	6
Volume Number	18
Language	English
Publisher	Informa UK Limited
Publisher Date	1988-01-01
Access Restriction	Open
Subject Keyword	Journal: Xenobiotica Cell Culture Cytotoxicity Metabolising
Content Type	Text
Subject	Health, Toxicology and Mutagenesis Medicine Biochemistry Toxicology Pharmacology

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