NDLI: Sensitivity enhancement for colorimetric glucose assays on whole blood by on-chip beam-guidance

Content Provider	Springer Nature Link
Author	Grumann, M. Steigert, J. Riegger, L. Moser, I. Enderle, B. Riebeseel, K. Urban, G. Zengerle, R. Ducrée, J.
Copyright Year	2006
Abstract	In this paper, we present a novel concept for optical beam-guidance to significantly enhance the sensitivity of colorimetric assays by extending the optical path length through the detection cell which linearly impacts the resulting attenuation of a probe beam according to the law of Beer-Lambert. In our setup, the incident probe beam is deflected by 90∘ into the chip plane at monolithically integrated V-grooves to pass a flat detection cell at its full width (i.e., with a path length of 10 mm) instead of its usually much smaller height. Afterwards, the attenuated beam is redirected by another V-groove towards an external detector. The general beam-guidance concept is demonstrated by a glucose assay on human whole blood on a centrifugal microfluidic “lab-on-a-disk” platform made of COC. We achieve an excellent linearity with a correlation coefficient (R 2) of 0.997 paired with a lower limit of detection (200 μM) and a good reproducibility with a coefficient of variation (CV) of 4.0% over nearly three orders of magnitude. With an accelerated sedimentation of cellular constituents by centrifugal forces, the sample of whole blood can be analyzed in a fully integrated fashion within 210 s. This time-to-result can even be improved by the numerical extrapolation of the saturation value. Additionally, the direct assay on whole blood also shows a negligible correlation with the hematocrit of the blood sample.
Starting Page	209
Ending Page	214
Page Count	6
File Format	PDF
ISSN	13872176
Journal	Biomedical Microdevices
Volume Number	8
Issue Number	3
e-ISSN	15728781
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2006-05-27
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Glucose Optical beam-guidance Lab-on-a-disk Colorimetric assay Total internal reflection Nanotechnology Biophysics/Biomedical Physics Engineering Fluid Dynamics Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering

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