NDLI: Integrated sample-to-detection chip for nucleic acid test assays

Content Provider	Springer Nature Link
Author	Prakash, R. Pabbaraju, K. Wong, S. Tellier, R. Kaler, K. V. I. S.
Copyright Year	2016
Abstract	Nucleic acid based diagnostic techniques are routinely used for the detection of infectious agents. Most of these assays rely on nucleic acid extraction platforms for the extraction and purification of nucleic acids and a separate real-time PCR platform for quantitative nucleic acid amplification tests (NATs). Several microfluidic lab on chip (LOC) technologies have been developed, where mechanical and chemical methods are used for the extraction and purification of nucleic acids. Microfluidic technologies have also been effectively utilized for chip based real-time PCR assays. However, there are few examples of microfluidic systems which have successfully integrated these two key processes. In this study, we have implemented an electro-actuation based LOC micro-device that leverages multi-frequency actuation of samples and reagents droplets for chip based nucleic acid extraction and real-time, reverse transcription (RT) PCR (qRT-PCR) amplification from clinical samples. Our prototype micro-device combines chemical lysis with electric field assisted isolation of nucleic acid in a four channel parallel processing scheme. Furthermore, a four channel parallel qRT-PCR amplification and detection assay is integrated to deliver the sample-to-detection NAT chip. The NAT chip combines dielectrophoresis and electrostatic/electrowetting actuation methods with resistive micro-heaters and temperature sensors to perform chip based integrated NATs. The two chip modules have been validated using different panels of clinical samples and their performance compared with standard platforms. This study has established that our integrated NAT chip system has a sensitivity and specificity comparable to that of the standard platforms while providing up to 10 fold reduction in sample/reagent volumes.
Starting Page	1
Ending Page	14
Page Count	14
File Format	PDF
ISSN	13872176
Journal	Biomedical Microdevices
Volume Number	18
Issue Number	3
e-ISSN	15728781
Language	English
Publisher	Springer US
Publisher Date	2016-05-11
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Lab-on-a-chip Nucleic acid tests Sample-to-detection Droplet microfluidics Dielectrophoresis Electrowetting Biomedical Engineering Biophysics and Biological Physics Nanotechnology Engineering Fluid Dynamics
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Integrated liquid and droplet dielectrophoresis for biochemical assays

DC-Dielectrophoretic separation of biological cells by size

A self-contained fully-enclosed microfluidic cartridge for lab on a chip

An integrated, self-contained microfluidic cassette for isolation, amplification, and detection of nucleic acids

Optofluidic Devices for Droplet and Cell Manipulation

Sample concentration and impedance detection on a microfluidic polymer chip

Integrated microfluidic biophotonic chip for laser induced fluorescence detection

Optimized acoustic biochip integrated with microfluidics for biomarkers detection in molecular diagnostics

Lab-on-a-chip for rapid electrochemical detection of nerve agent Sarin

Integrated sample-to-detection chip for nucleic acid test assays

Similar Documents

Integrated liquid and droplet dielectrophoresis for biochemical assays

DC-Dielectrophoretic separation of biological cells by size

A self-contained fully-enclosed microfluidic cartridge for lab on a chip

An integrated, self-contained microfluidic cassette for isolation, amplification, and detection of nucleic acids

Optofluidic Devices for Droplet and Cell Manipulation

Sample concentration and impedance detection on a microfluidic polymer chip

Integrated microfluidic biophotonic chip for laser induced fluorescence detection

Optimized acoustic biochip integrated with microfluidics for biomarkers detection in molecular diagnostics

Lab-on-a-chip for rapid electrochemical detection of nerve agent Sarin

Integrated sample-to-detection chip for nucleic acid test assays