NDLI: Duffy blood group (Fya & Fyb) analysis using surface plasmon resonance

Content Provider	Springer Nature Link
Author	Then, Whui Lyn McLiesh, Heather Aguilar, Marie Isabel Garnier, Gil
Copyright Year	2016
Abstract	The most widely known blood groups, ABO and RhD, have been extensively observed as having strong antibody-antigen interactions during blood typing. However, not all interactions show the same binding affinity. The Duffy blood group system, where Fya and Fyb antigens are the most clinically significant, are only available with an IgG antibody structure, and display weak binding interactions. While current blood typing techniques are well established, methods for quantifying the binding strength are more limited. Surface Plasmon Resonance (SPR) provides avenues for developing more robust detection methods, and serve as a sensitive quantification technique by itself. This study tested SPR for the detection of weaker antibody-antigen interactions using the Duffy blood groups, Fya and Fyb, as a model. This study shows a minimum threshold of antibody concentration is required for successful detection. Some instances of detection were successful using concentrated commercial anti-Fya and anti-Fyb solution during the incubation stage. However, these results were not fully reproducible. We found that a significant dissociation of the Duffy antigen-antibody complex occurs over time. A combination of factors affects the detection of the Duffy antigens using SPR; these include antibody concentration, antigen expression, and antigen structure. This results in weak, unstable and reversible antibody-antigen interactions which are currently limiting accurate and reproducible detection by SPR. Despite these issues, detection of Duffy antigens Fya and Fyb was demonstrated using SPR; however, further development is required for SPR to become a robust clinical blood typing technique.
Starting Page	1
Ending Page	9
Page Count	9
File Format	PDF
ISSN	13872176
Journal	Biomedical Microdevices
Volume Number	18
Issue Number	6
e-ISSN	15728781
Language	English
Publisher	Springer US
Publisher Date	2016-10-27
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Surface plasmon resonance Blood group typing Antibody-antigen interactions Weak variants Anti-human IgG Biomedical Engineering Biological and Medical Physics, Biophysics Nanotechnology Engineering Fluid Dynamics
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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