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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Then, Whui Lyn Aguilar, Marie-Isabel Garnier, Gil |
| Description | Country affiliation: Australia Author Affiliation: Then WL ( Bioresource Research Institute of Australia (BioPRIA), Australian Pulp and Paper Institute (APPI), Department of Chemical Engineering, Faculty of Engineering, Monash University, Clayton, VIC 3800, Australia.); Aguilar MI ( Department of Biochemistry and Molecular Biology, Faculty of Medicine, Nursing and Heath Sciences, Monash University, Clayton, VIC 3800, Australia.); Garnier G ( Bioresource Research Institute of Australia (BioPRIA), Australian Pulp and Paper Institute (APPI), Department of Chemical Engineering, Faculty of Engineering, Monash University, Clayton, VIC 3800, Australia. Electronic address: gil.garnier@monash.edu.) |
| Abstract | The accurate and reliable typing of blood groups is essential prior to blood transfusion. While current blood typing methods are well established, results are subjective and heavily reliant on analysis by trained personnel. Techniques for quantifying blood group antibody-antigen interactions are also very limited. Many biosensing systems rely on surface plasmon resonance (SPR) detection to quantify biomolecular interactions. While SPR has been widely used for characterizing antibody-antigen interactions, measuring antibody interactions with whole cells is significantly less common. Previous studies utilized SPR for blood group antigen detection, however, showed poor regeneration causing loss of functionality after a single use. In this study, a fully regenerable, multi-functional platform for quantitative blood group typing via SPR detection is achieved by immobilizing anti-human IgG antibody to the sensor surface, which binds to the Fc region of human IgG antibodies. The surface becomes an interchangeable platform capable of quantifying the blood group interactions between red blood cells (RBCs) and IgG antibodies. As with indirect antiglobulin tests (IAT), which use IgG antibodies for detection, IgG antibodies are initially incubated with RBCs. This facilitates binding to the immobilized monolayer and allows for quantitative blood group detection. Using the D-antigen as an example, a clear distinction between positive (>500 RU) and negative (<100 RU) RBCs is achieved using anti-D IgG. Complete regeneration of the anti-human IgG surface is also successful, showing negligible degradation of the surface after more than 100 regenerations. This novel approach is validated with human-sourced whole blood samples to demonstrate an interesting alternative for quantitative blood grouping using SPR analysis. |
| ISSN | 09565663 |
| Volume Number | 73 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-11-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Blood Grouping And Crossmatching Methods Surface Plasmon Resonance Antibodies, Immobilized Immunology Statistics & Numerical Data Erythrocytes Humans Immunoglobulin Fc Fragments Immunoglobulin G Rh-hr Blood-group System Rho(d) Immune Globulin Journal Article Research Support, Non-u.s. Gov't Validation Studies Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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